Lecture two

Question 1

Distinguish between observation and hypothesis

Answer 1

Observation based – quantatative, qualitative, must be repeatable – inductive - specific to general
Hypothesis based – forming and testing a hypothesis, deductive reasoning general to specific

Question 2

Cell theory

Answer 2

Cells are the basic units of life
All living organisms are composed of cells
Cells arise by division of pre-existing cells

Question 3

Distinguish between prokaryotic cell size and eukaryote cell size

Answer 3

10 micrometers to 100 micrometers in diameter (1000 micrometer = 1mm) Eukaryotes
1 micrometer

Question 4

Distinguish between hypotheses and theories in science

Answer 4

Hypothesis – a possible explanation of an observation, must be both testable and falsifiable
Scientific theory – a broad explanation supported by many observations or experiments

Question 5

Describe light microscopy

Answer 5

Light passes through specimen to produce image. Light focused using glass lens

Question 6

Fluorescence microscopy

Answer 6

UV light is used as a light source. Dyes are used to stain cells. It uses fluorescence to highlight structures in fixed and living biological specimens instead of using absorption, phase or interference effects.

Question 7

Confocal microscopy

Answer 7

Can see cells in 3D.