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Hematology 2 > Lecture: Lab Eval of Hemostasis > Flashcards

Lecture: Lab Eval of Hemostasis Flashcards

1
Q

Important in collagen synthesis

A

Vitamin C or ascorbate

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2
Q

In Hess Test Positive Pressure Technique, BP cuff is inflated to _ for _ minutes

A

80mmHg
8 minutes

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3
Q

Positive results of Hess Test Positive Pressure?

A

Presence of petechiae

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4
Q

In Negative Pressure Technique, a modified _ instrument is used to inflate at _ for _ minute

A

da Silva Melle
200mmHg
1 minute

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5
Q

Normal value of petechiae in Negative Pressure Method

A

Less than 4 petechiae

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6
Q

Normal value of petechiae in Rumple-Leede

A

0-10

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7
Q

Abnormal value of petechiae in Rumple -Leede

A

More than 20

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8
Q

Equivocal value of petechiae in Rumple-Leede

A

10-20

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9
Q

Composition of Reese and Eckers’

A

Citrate
Formaldehyde
Buffer
BCB

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10
Q

Dilution factor of RBC in Toncantin’s

A

1:200

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11
Q

Dilution factor of WBC in Toncantin’s

A

1:20

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12
Q

Direct Method is also known as

A

Reese and Ecker’s
Toncantin’s

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13
Q

Unopette System contains?

A

Ammonium oxalate

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14
Q

Dilution factor utilized in Unopette System

A

1:100

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15
Q

In Fonio’s Method, 10-40 RBC equates to _ platelet in its indirect counting estimation

A

1 platelet

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16
Q

In Fonio’s Method, 100 RBC equates to _ platelet in its indirect counting estimation

A

3-10 platelets

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17
Q

In Fonio’s Method, 200 RBC equates to _ platelet in its indirect counting estimation

A

5-20 platelets

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18
Q

0-49,000/uL is reported as?

A

Marked Decrease

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19
Q

50,000-99,000/uL is reported as?

A

Moderate Decrease

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20
Q

100,000-149,000/uL is reported as?

A

Slight Decrease

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21
Q

150,000-199,000/uL is reported as?

A

Low Normal

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22
Q

200,000-400,000/uL is reported as?

A

Normal

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23
Q

401,000-599,000/uL is reported as?

A

Slight Increase

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24
Q

600,000-800,000/uL is reported as?

A

Moderate Increase

25
Q

Above 800,000/uL is reported as?

A

Marked Increase

26
Q

These disorders are what type of thrombocytopenia?

Idiopathic thrombocytopenic purpura (ITP)
Post-transfusion
Heparin-induced

A

Primary Thrombocytopenia

27
Q

These disorders are what type of thrombocytopenia?

Systemic lupus erythematosus (SLE)
Viral infection
Lymphoproliferative disorders

A

Secondary Thrombocytopenia

28
Q

An abnormal increase in the number of circulating platelets as a result of physiologic or pathologic processes

A

Thrombocytosis

29
Q

These disorders are what type of thrombocytosis?

Myelodysplastic Syndromes (MDS) such polycythemia vera and essential thrombocythemia

A

Primary Thrombocytosis

30
Q

These disorders are what type of thrombocytosis?

After hemorrhage
IDA
Malignancy
Epinephrine administration

A

Secondary Thrombocytosis

31
Q

In vivo platelet function and number test

A

Bleeding Time

32
Q

Subjective pressure
More practical and convenient
Finger pricking and earlobes

A

Duke’s Method

33
Q

Better method based on its principle
Puncture forearm with blood pressure cuff

A

Ivy’s Method

34
Q

Cuff is inflated to _ in Ivy’s Method

35
Q

Almost the same with Duke’s but the
difference is immersion of puncture finger in sterile physiologic saline solution (NSS) warmed at 37C water bath

A

Adelson-Crosby Method

36
Q

Same as Adelson-Crosby Method

A

Cody-Lalitch

37
Q

Same principle as Adelson-Crosby Method but it only uses earlobe as the site of puncture

A

MacFarlane for Bleeding Time

38
Q

Assesses the effect of a standard dose of aspirin on the Duke’s Bleeding Time

A

Aspirin Tolerance Test

39
Q

In vitro determination of platelet adhesiveness

A

Platelet Retention Test (Glassbead Retention Test)

40
Q

These disorders are abnormally decreased or abnormally increased in platelet retention test?

Bernard-Soulier
Glanzmann Thrombastenia
vWD
Chediak-Higashi
Myeloproliferative
Disorder
Uremia
Aspirin Administration

41
Q

These disorders are abnormally decreased or abnormally increased in platelet retention test?

Thrombotic Disorders
Hyperlipidemia
Carcinoma
Oral Contraceptives
Pregnancy

42
Q

Principle of Platelet Aggregation Evaluation

A

Turbidimetry / Transmittance of Light

43
Q

What disorder is it where upon adding ristocetin, it fails to aggregate?

A

Bernard-Soulier Syndrome

44
Q

What disorder is it when adding ECA, it abnormally reacts?

A

Glanzmann Thrombasthenia

45
Q

Too low platelet, high transmittance

A

PPP (protein-poor plasma)

46
Q

Too high platelet, low transmittance

A

PRP (protein-rich plasma)

47
Q

Anticoagulant used in testing platelet aggregation

A

Citrated blue top

48
Q

Last added aggregating agent

A

Epinephrine

49
Q

First to be added and has broad curve

A

Ristocetin

50
Q

Aggregating agents that cause biphasic curve

A

Epineprhine
Thrombin
ADP

51
Q

Weak aggregating agent; 30% T

52
Q

It reflects the number and quality of platelets, fibrinogen concentration, the fibrinolytic system and the packed cell
volume

A

Clot Retraction

53
Q

Using castor oil for constipation; (+) dimpling phenomenon for 15-45 minutes; Mickey mouse appearance

A

Hirschboeck Method

54
Q

Blood is allowed to clot in a test tube containing a glass rod and retraction is observed after the incubation period. The clot attached to the rod is then removed and the serum extracted
from the clot is measured.

A

MacFarlane for Clot Retraction

55
Q

Normal Value for MacFarlane for Clot Retraction

A

44-67% for both methods

56
Q

Blood in a test tube used for clotting time (one of the 3 tubes in the Lee and White Method is used) is saved and left at room temperature in order to note retraction, red cell fall out and clot
lyses

A

Single Tube Method

57
Q

Measures clotting time then compare the result, promote clotting

If PRP is near control, normal
If PPP is near control, abnormal

A

PF3 Availability

58
Q

Platelet are activated, change shape, release granules; Presence means on going platelet activation; Method is radioimmunoassay or RIA

A

PF4 and BTG

Hematology 2 (6 decks)

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