Laboratory: Platelet Count Flashcards
Reservoir of platelet in man
Spleen
Indirect Methods of Platelet Determination
Fonio’s Method
Dameshek Method
Olef’s Method
Direct Methods of Platelet Determination
Guy and Leake
Reese and Ecker’s
Brecker-Cronkite
Platelets are counted in RBC bath. The voltage drops after every passage of platelet in the aperture
Voltage-Pulse Counting
Employs a sensitive photodiode, laser light and high angle to count platelets in the presence of RBC
Light Scattering
Wright’s stain; Diluting fluid is 14% MgSO4
Fonio’s Method
Diluting fluid of Fonio’s Method is?
14% MgSO4
Stain used in Dameshek’s Method
Brilliant Cresyl Blue
Dilution Factor used in Dameshek’s Method
1:5
The indirect count can be checked based on the examination done of the blood smear
Olef’s Method
Decreased in number of platelet
<1 plt/OIF
Adequate amount of platelets
5-20 plt/OIF
Increase in the number of platelet
> 25 plt/OIF
Diluent: sodium citrate, 40% formalin and crystal violet
Guy and Leake’s
Diluent: Na Citrate, brilliant cresyl blue, formalin, and distilled H2O
Rees and Ecker’s
Reference method; Uses phase-contrast microscope; Diluent is 1% ammonium oxalate
Brecker- Cronkite
Diluent: sodium oxalate; reported in percent
Van-Allen
Consists of reservoir enclosed by a diaphragm, a pipette attached to an overflow chamber, a pipette shield; The reservoir contains a specific volume of diluting fluid, 1.98 mL of 1% buffered ammonium oxalate (1:100)
Unopette
Finger is punctured 3mm deep; Blot drop every with filter paper every 30 seconds
Duke’s Method
Normal Range for Duke’s Method
2 to 4 minutes
Uses sphygmomanometer (inflated to 40 mmHg); Make 2 skin incision (2mm deep, 2mm long) avoiding any subcutaneous vein
Ivy Method
Normal Range of Ivy Method
1 to 7 minutes
Uses a template containing a standardized slit in place of the disposable lancets
Template Bleeding Time
Normal/Reference Range for Template Bleeding Time
2 to 9 minutes
An example of capillary fragility test (tourniquet test); Determine the patient’s blood pressure
Rumple-Leed Test
0-10 petechiae is graded as?
1+
10-20 petechiae is graded as?
2+
20-50 petechiae is graded as?
3+
50 and above petechiae is graded as?
4+
Observe the extrusion of droplet like serum “dimpling” on top portion of the drop of blood
Hirschboeck/Castor Oil
