Lecture: Introduction to Virology Flashcards
Is virus cellular or acellular?
Acellular
Diameter range of viruses
20 nm to 450 nm
What type of microscope can view a virus?
Transmission electron microscope
What is shown when you view a virus in a Bright-field microscope?
Effect of the virus
Viruses are incapable of metabolism, growth, and reproduction. They also only replicate inside the cells, therefore they are?
Obligate intracellular parasites
Can a virus contain both DNA and RNA?
No, either DNA or RNA only
Viruses have cell walls, cell membranes, or cellular organelles. True or False?
False, they do not have cellular structure
Known as the complete structure/infective form of a virus
Virions
Viruses lack the enzyme for protein and nucleic acid synthesis. True or False?
True
Are viruses prokaryotic or eukaryotic?
Neither
All viruses have envelopes, but not all have capsids. True or False?
False, all viruses have capsids but not all have envelopes
A virus without an envelope is called?
Naked Virus
Responsible for the introduction of viral nucleic acid into a suitable host cell
Viral attachment protein
Formed when the viral particle carries off a part of the host cell’s membrane
Envelope
Which is more susceptible to environmental conditions, Naked or Enveloped?
Enveloped. Naked viruses are more resistant to environmental conditions
Protein spikes protruding through the envelope from the capsid
Spikes / Peplomers
Outer protective shell; protect genetic material of virus; most prominent geometric feature
Capsid
Capsids are composed of identical protein subunits known as
Capsomeres
Three Major Capsid Structures
Helical
Icosahedral
Complex
Double-stranded DNA viruses are what class?
Class I
Single-stranded DNA viruses are what class?
Class II
Double-stranded RNA viruses are what class?
Class III
Positive-sense, single-stranded RNA viruses are what class?
Class IV
Negative-sense, single-stranded RNA viruses are what class?
Class V
RNA viruses that reverse transcribe (HIV) are what class?
Class VI
DNA viruses that reverse transcribe (HBV) are what class?
Class VII
Class IV starts with an mRNA which proceeds to translation and production of viral proteins. True or False?
True
Class V also starts with mRNA then is converted to complementary mRNA then translation and production of proteins. True or False?
False, Class V starts with a complementary mRNA being converted into mRNA which proceeds to translation and production of proteins
Class VI uses reverse transcription when RNA from central dogma needs to be transcribed into DNA to infect more cells. True or False?
False, it is Class VII that uses that kind of process for RT
Class VII uses reverse transcription to become DNA and join central dogma via entering the nucleus. True or False?
False, it is Class VI that uses that kind of process for RT
Ending of -virales is what order of taxonomy?
Orders
Ending of -viridae is what order of taxonomy?
Family
Ending with -virinae is what order of taxonomy?
Subfamily
Ending with -virus is what order of taxonomy?
Genera
The correct chronological order of viral replication?
Attachment - Penetration - Uncoating - Synthesis/Replication - Assembly - Maturation - Release
What step is skipped when a bacteriophage is infecting a cell?
Uncoating
Live-Attenuated vaccines have?
Weakened organism
Inactivated vaccines have?
Killed/dead organism
One or more purified components of a pathogen is contained in what vaccine?
Subunit
Bacterial toxins that have been chemically inactivated so that they are not pathogenic is in what vaccine?
Toxoid
Purified Component Vaccine is composed of what?
Biochemically purified components of a microorganism
Polysaccharide Vaccine is composed of what?
Biochemically purified polysaccharide from bacterial capsule
Protein produced by genetically-modified non-pathogenic bacteria, yeast or other cells is contained in what vaccine?
Recombinant
Circular single-stranded RNA with some pairing between base and loops where no such pairing occurs
Viroids
Misfolded proteins that affect CNS
Prions
Baltimore Classification System classifies viruses based on their?
Type of nucleic acid genome
Replication strategy of the virus
What examples of viruses have complex capsid structure?
Poxviruses
Bacteriophage
Drugs that disrupts the uncoating?
Amantadine
Rimantadine
Drug that inhibits attachment?
Maraviroc
Drugs that inhibit viral replication?
INF-alpha
Ribavirin
Drugs that inhibits reverse transcriptase?
ddl
ABC
3TC
d4T
ddC
FTC
TDF
Drugs that inhibit proteases?
Indinavir
Nelfinavir
Ritonavir
Saquinavir
Drugs that inhibit DNA polymerase?
Acyclovir
Cidofovir
Famciclovir
Ganciclovir
Valacyclovir
Drugs that inhibits DNA synthesis/DNA base catalogue?
Idoxuridine
Trifluridine
Drug that inhibits adenosine catalog?
Remdesivir
Drugs that are active against chronic HBV?
Lamivudine
Adefovir
Drugs that inhibits/disrupts release?
Oseltamivir
Zanamivir
Peramivir
Drugs that inhibits/disrupts penetration?
Enfuvirtide
Docosanol
HIV integrase inhibitors
Raltegravir
Elvitegravir
Dolutegravir
ICTV means?
International Committee on Taxonomy of Virus