Lecture 7 Flashcards
What are the main areas of molecular testing
Inherited disorders, acquired disease, infectious disease, and genetic predisposition syndromes.
What are some applications of PCR in infectious disease
Rapid diagnostics for hospital-acquired infections (e.g., MRSA, C. difficile) and viral load monitoring for HIV or HCV.
What is LAMP
: Loop-mediated isothermal amplification, a rapid and sensitive method using 4-6 primers to amplify target DNA at a single temperature (63-67°C).
What is NEAR?
Nicking enzyme amplification reaction, a rapid and sensitive method using a strand-displacing DNA polymerase and a nicking enzyme to produce short nucleic acids from the target sequence
: What is the Alere i Influenza test
: A CLIA-waived test using NEAR technology to detect RNA gene targets from influenza A and B viruses with a turnaround time of 15 minutes.
What is the Cobas® Liat platform
A platform using real-time PCR to detect bacterial DNA or viral RNA targets, with tests for SARS-CoV-2, Influenza A/B, RSV, Group A Strep, and C. difficile.
What is CRISPR
A bacterial immune system mechanism using repeating sequences of genetic code and spacer sequences to detect and destroy invading viruses.
How is CRISPR used in diagnostics
Using a CRISPR Cas12a/gRNA complex and a fluorescent probe to detect target amplicons produced by RT-PCR or isothermal RPA.
What are TaqMan Array Cards
A microfluidic platform for multiplex real-time PCR, allowing 384 simultaneous PCRs with customizable assay targets
What are the future trends in molecular POC
Rapid innovation in genetic engineering, gene editing, gene therapy, expansion of CLIA-waived molecular POCT, and improved microfluidic and miniaturized platforms.
