Lecture 6 Enzymes Flashcards
Define the following terms:
-Catalyst
A substance which will increase the speed of the chem rnx
Define the following terms:
-Substrate
The molecule undergoing RXN
Define the following terms:
-Ligand
A molecule that bings to cause conformational change
Define the following terms:
-Enzyme
Catalyst if living organisms
Define the following terms:
-Enzyme product complex(EP)
The complex where the enzyme forms the product
Define the following terms:
-Product
What comes out of substrate and enzyme
Define the following terms:
-Stereoisomer
Same chemical formula but differ over a chirality
Define the following terms:
-Activation Energy
The energy required to reach transitional state
Define the following terms:
-Transitional State
The point of maximal free engird
Define the following terms:
-Activation energy barrier
The energy required to reach the transitional state, IE the AE
Understand the mechanisms involved substrate specificity including:
-Catalysis by proximity
When an enzymes active site will position molecule within bonding forming distance.
Understand the mechanisms involved substrate specificity including:
-Induced fir model or catalysis by strain
The enzyme changes shape upon binding to the substrate
Understand the mechanisms involved substrate specificity including:
-Acid base catalysis
AA side chains in enzyme Activation site can transfer Protons between enzymes, substrates, transitional states and other intermediates
accelerates the reaction
Understand the mechanisms involved substrate specificity including:
-Transitional state stabilization
enzymes bind the substate in geometry which resembles the transitional state which will stabilize the transitional state
promoting formation and accelerate rxn
Be able to interpret reaction coordinate diagrams
Plots the free energy
Explain factors that make enzyme reactions specific
BINDS CHEMISTRY then DISSOCIATION
Understand and explain
-basis for rate rxn
A substate will have increase rate of rxn w/ attached enzyme(catalyst of living organism)
Understand and explain that enzymes do not change eq point
enzymes only change AE and do not effect how much will be procured only how fast they will be produced.
How do enzymes convert non-chiral substrates to specific stereoisomers
by moving one of the sides to a different position( up or down)
List the types of catalysis in chymotrypsin
Identify the catalytic triad and delineate their roles in catalysis
Understand the steps in catalysis
-General base catalysis, Covalent Catalysis, and transitional state stabilization
-ASP102, HIS 57, and SER 195
ASP102 Polarizes HIS 5 7 making able to accept electrons
HIS 57 accepts electrons
SER 195 donates electrons
How do enzymes accelerates rate of rxn w/o affecting KEQ
There is no change in the delta g of a reaction. if there was then there would be a change in the keq
