Lecture 6: dao

Question 1

what can biofilms cause in dentistry

Answer 1

peridontal disease

Question 2

what can biofilms cause in microbial engineering

Answer 2

-biofouling, waste water treatment and biomediatiopm

Question 3

biofilms cause over… of hospital aquired infections

Answer 3

65%

Question 4

where are biofilms in hospitals:

Answer 4

medical devices, catheters and implants

Question 5

what can biofilms cause in a heart valve

Answer 5

endocarditis

Question 6

true or false: biofilms can cause biofilms

Answer 6

true

Question 7

biofilms in people are due to….

Answer 7

chronic pr subacute infections

Question 8

what are the 6 main points in biofilms

Answer 8

-multicellular bacterial communities
-extracellular matrix (glue that binds it all)
-non-motile cells attached to a surface or to each other
-physiologic heterogeneity (all bacteria will differenciate a tad from everyone else)
-bacterial communication
-specialized subpopulations

Question 9

where is b subtilis from?

Answer 9

-soil organism and gut commensal

Question 10

which bacteria is a potential beneficial bacteria for plants

Answer 10

b subtilis

Question 11

true ot false: b subtilis is a poor biofilm former

Answer 11

false it is a good one

Question 12

staphylococcus aureus is a major pathogen of the ……

Answer 12

skin, soft tissue and foreign body infections

Question 13

staph aureus is highly adherent to what

Answer 13

to abiotic and mucosal surfaces

Question 14

true or false: staph aureus is a foreign body expert

Answer 14

true

Question 15

pseudomonas aeruginosa is an important …. pathogen

Answer 15

fibrosis

Question 16

which bacteria causes lethal infection

Answer 16

peudomonas aeruginosas it forms complex biofilm structures

Question 17

where is pseudomonas aeruginosas found

Answer 17

in the environment

Question 18

true or false: e.coli is an uti pathogen

Answer 18

true

Question 19

Which statements are true about biofilms? select ALL that apply
* biofilm growth requires a few specific genes that are rare among pathogens
* biofilm cells behave differently from planktonic bacteria
* antibiotics are made by bacteria growing as biofilms
* biofilm cells are less susceptible to antibiotics and immune defenses
* all of you have touched biofilms with your fingers

Answer 19

• biofilm cells behave differently from planktonic bacteria
• biofilm cells are less susceptible to antibiotics and immune defenses
• all of you have touched biofilms with your fingers

Question 20

Abiotic adherent biofilms

Answer 20

• Calgary device, 96-well plates, Biofilm adherent to polystyrene surface
• Growth under static conditions
• Quantification of biofilm biomass after staining with crystal violet
• Easy, high throughput
• Limited biomass and architecture, highly attachment dependent

Question 21

pedicle biofilms

Answer 21

• auto-aggregated cell clusters, typically at the air-liquid interface
• Bacterial are mostly adherent to each other, with a few adherent to side surfaces
• Easy, but poorly quantitative
• Few species (B. subtilis) develop structured biofilms

Question 22

in vitro biofilm models: flow cell biofilms

Answer 22

• Flow cell chambers
• Visualization by confocal microscopy
• Under continuous flow (shear stress)
• Complex biofilm architecture
• Low throughput and complex

Question 23

in vitro biofilm models: microcolony or gel biofilms

Answer 23

• Under static conditions in semi-solid media
• Bacteria are primarily adherent to each other
• Simple, reproducible and quantitative
• Colony biofilms on agar or membranes
• Limited imaging
• Complex architecture in some species, strains and conditions

Question 24

biofilm devlopment

Answer 24

-initial attachment
-irreversible attachment and cell replication
-early biofilm formation
-biofilm maturation
-biofilm dispersal