Lecture 6

Question 1

What is Src? what is it’s role?

Answer 1

Non-receptor protein kinase, regulating cellular processes such as cell proliferation and migration

Question 2

What do the SH2 and SH3 of Src bind to?

Answer 2

SH2 binds to phosphotyrosine residues
SH3 domain interacts with a poly-proline moti

Question 3

how do you activate Src Kinase

Answer 3

dephosphorylation of Tyr530 followed by autophosphorylation at Tyr419

Question 4

How is active Src deactivated

Answer 4

rephopshorylation of Tyr530 by C-terminal Src kinase

Question 5

how does tyrosine phosphorylation of the C-terminus of Src affect it

Answer 5

inhibits the interaction

Question 6

How would the Rous virus affect Src?

Answer 6

Would remove the tyr527 terminus so there would be no way of inhibiting Src, Src would always be on

Question 7

what is myristoylation

Answer 7

lipid modification involving the addition of myristic acid to the N-terminal glycine of a subset of proteins

Question 8

what is the function of BCR? what does BCR stand for

Answer 8

Breakpoint cluster region, two opposing regulatory activities towards GTP-binding proteins
C-terminus is GTPase activating which stimulates GTP hydrolysis of RAC-GTP
Central Dbl homology domain functions as guanine exchange factor, promotes the conversion of RAC-GDP to RAC GTP
positive and negative regulators of RAC1 activity

Question 9

How does BCR affect Abl

Answer 9

When Bcr binds to Abl, it is activated constitutively.

Question 10

Describe the functions of protein kinases and phosphatases

Answer 10

kinase: adds phosphate (ATP is used)
Phosphatase: removes phosphate (Free phosphate is generated)

Question 11

Study diagrams for Src and Abl