Lecture 6 - 1/12 (Gluconeogenesis)

Question 1

Biotin

Answer 1

carries CO2 for carboxylation

Question 2

transanimation

Answer 2

moving amine group (e.g. from pyruvate to alanine)

Question 3

Glucose phosphatase

Answer 3

Used in last step of gluconeogenesis and only happens in the liver because that’s the only place the gene for glucose 6-phosphatase is expressed. It is in the liver (and kidney) cells ER lumen.

Question 4

Oxaloacetate

Answer 4

4-C transitionary state between pyruvate and PEP. reduced to malate in order to be moved to the cytoplasm.

Question 5

Pyruvate carboxylate

Answer 5

Not in the cytoplasm. Only in the mitochondria. So must shuttle pyruvate into the mitochondrial matrix to do carboxylation.

Question 6

Pyruvate dehydrogenase

Answer 6

Multiple subunits. carries out the oxidation of pyruvate.

1. decarboxylation
2. oxidation
3. transfer to CoA