Lecture 5: Lipid Metabolism Flashcards
What is the major source of carbon for FA synthesis? What are the precursor and end product of FA Synthesis
major source: dietary carbohydrates
FA Synth precursor: Acetyl CoA
FA Synth endproduct: palmitic acid
What are the 3 phases of Fatty Acid Synthesis?
Phase 1: Cytosolic Entry of ACoA
Phase 2: Generation of Malonyl CoA
Phase 3: FA chain formation
FA Synth Phase I Enzymes
- Citrate Synthase
- oxaloacetate and ACoA = citrate
- ATP Citrate Lyase
(+) - glucose, insulin
(-) - PUFA, leptin
FA Synth Phase II Enzymes
- Acetyl CoA carboxylase (adds CO2 to ACoA)
(+) - citrate, insulin
(-) - glucagon, epinephrine, AMP, palmitate, PUFA
- RATE LIMITING ENZYME (needs BIOTIN)
- 2C converted to 3C
What does malonyl CoA regulate?
- inhibits carnitine acyltransferase (FA degradation RLS)
- prevents synthesis and degradation from occurring simultaneously
FA Synth Phase III Enzyme
- Fatty Acid Synthase
(+) - insuling, glucocorticoid hormones
(-) - PUFA
multi-enzyme complex, 2 identical dimers, head to tail
- Acyl Carrier Protein (ACP) moves things between each of the 7 enzymes on one half of the dimer
Fatty Acid Synthesis Reactions
- Condensation (acetyl and malonyl groups)
- Reduction (ketoacyl –> hydroxyl)
- Dehydration (hydroxyl –> trans-enone)
- Reduction (4 chain fatty acyl group)
repeats 6 more times
Regulation of Fatty Acid Synthase (allosteric and induction/repression at gene level)
Allosteric:
(+) - phosphorylated sugars
Gene Level:
(+) - insulin, glucocorticoid hormones, hi carb/low fat
(-) - high fat diets, PUFA
Synthesis of Longer Chain FA and carbon donors
- elongation in SMOOTH ENDOPLASMIC RETICULUM
- lengthened 2 carbons at time (NADPH reducing power)
SER –> Malonyl CoA (carbon donor)
Mitochondria –> Acetyl CoA (carbon donor)
FA Desaturation
- Acyl CoA Desaturases (4 desaturases - 4,5,6,9)
cannot introduce DB past carbon 9 and 10
- require omega 3 and 6 fatty acids (Essential FA)
Essential Fatty Acids (2) and what they can make
Omega 6: Linoleic Acid (18:2)
- can make arachidonic acid (20:4)
- precursors for EICOSANOIDS
Omega 3: Linolenic Acid (18:3)
- can make EPA (20:5) and DHA (22:6)
TAGs and Energy Storage
- TAGS contain 6.75 times as much energy as glycogen (carbs)
normal man: 40 Cal glucose, 600 Cal glycogen, 24,000 Cal protien, 100,000 Cal TAGs
3 main sources of TAGs
- Dietary TAGs (intestinal processing)
2. De Novo TAGs (hepatocytes and adipocytes)
Intestinal TAG Synthesis
- dietary TAGS –> MAG and FFA in intestinal lumen
- MAG used as backbone (2 fatty acyl CoA added) in order to make TAG
- TAGs packaged w/apolipoproteins and other lipids to make Chylomicrons (released to lymphatic system and enter blood)
Hepatocyte TAG Synthesis and enzymes
enzymes: fatty acid acyl synthetase, G3P dehydrogenase, glycerol kinase
- glucose/glycerol form G3P (used as TAG backbone)
- FFA (liver) added to G3P to form TAGs
- packaged w/apolipoproteins/lipids to make VLDL (released into bloodstream)