Lecture 5: Imaging techniques

Question 1

Haematoxylin: Stains _______ dark purple

Periodic Acid- Schiff Reaction: Stains ________ magenta

Answer 1

Nucleic Acids

Carbohydrates

Question 2

______________ is a commonly immunohistochemical tecnique. metalloenzyme that catalyzes the oxidation of various organic substrates by hydrogen peroxide, turning them BROWN

Answer 2

Horse Radish Peroxidase

Question 3

Describe Fluresence Microscopy

Answer 3

Question 4

The _______ can be changed in FLourescent microscoopy to visualize different wavelengths

Answer 4

Filter

Question 5

___________uses a laser and pinhole aperture to scan a specimen at a specific depth

Answer 5

Confocal Microscopy

Question 6

The specimen must be coated in a thin film of heavy metal for this technique. Scattering of electrons as a beam of electrons hits each point is recorded by the detector building image of 3D shapes.

Answer 6

Scanning Electron Microscope

Question 7

Electron scanning technique that requires the specimen to be very thin

Answer 7

Transmission Electron Microscopy

Question 8

How to study an electron micrograph:

1. Note the _______ to keep a sense of proportion.
2. The size of the _______ is a useful reference with respect to other organelles.
3. Identify the plasma membrane, it defines the limits of the cell.
4. _________ are often easy to identify.
5. The number of ______ can often tell you the function of the cell and the identity of the tissue.

Answer 8

How to study an electron micrograph:

1. Note the scale bar to keep a sense of proportion.
2. The size of the nucleus is a useful reference with respect to other organelles.
3. Identify the plasma membrane, it defines the limits of the cell.
4. Mitochondria are often easy to identify.
5. The number of organelles can often tell you the function of the cell and the identity of the tissue.

Question 9

__________ are actually course granules of several substances such as lipid (fat), glycogen (polysaccharide), zymogen(digestive proteins, e.g.enzymes), melanin (pigment) or others.

Answer 9

Cell Inclusions

Question 10

__________ have a dense wall and a hollow core and grow via polymeri-sation. They are found throughout the cell. Microfilaments

Answer 10

Microtubules

Question 11

__________ are found in thin sheets just below the plasma membrane which may be covered on its outer surface by a ________, a filamentous coat of glycoproteins

Answer 11

Microfilaments

Glycocalyx

Question 12

The space between the nucleus and nuclear envelope is called the __________

The circular gap formed when the inner and outer membrane fuse is called the nuclear ______

Answer 12

Perinuclear Cisterna

Nuclear Pore