Lecture 5

Question 1

What are pseudomons?

Answer 1

Small Gram negative rods with single polar flagelleum .

• unbiqutous and colnizes plants and animals.
• pseudomons are important decomposers and bioremediators.
• Use oxidase and catalase
• pigmented

Question 2

Whats the most important characteristic of pseudomons?

Answer 2

Theyre decomposers and they do not ferment carboyhydrates and use aeroic respiration.

-they produce acid from carbohydates but only in the presence of oxygen,

Question 3

Pseudomonas aeruginosa clincal importance:

Answer 3

• Commonly live in soil and water.
• Theyre part of normal flora of 10% of people.
• Contaminat IV and ventilators and anesthesia equipment.
• Resistant to soap, dyes, drugs and drying

MOST IMPORTANTLY THEYRE OPPURTUNISTIC PATHOGENS (for example burn victims get it)

Question 4

What makes pseudomonas an oppurtunisitc pathogen>

Answer 4

Because it usually targets burn victims.

• Nosocomial
• Causes skin and eye irratation
• common rrspiratory problems with patients of cystic fibrosis

Question 5

What are some pseudomons aeruginosa infections?

Answer 5

• Pulmonary infections topatients with cystic fibrosis and external otits.
• To treat use antibiotics or hyperimmune globulin.

Question 6

Why does Cystic fbrosis allows pseudomones areguinosa to thrive?

Answer 6

-Because cystic fibrosis causes hypersecretion of mucus.
PA flourishes in thcik membrane to grow plaque a& the plaque growth causes the attraction of neutrophiles and causes inflammation.

Question 7

What are the virulence detereminents of PA?

Answer 7

• LPS.
• Alginate
• Flagella
• Pilli
• Exotoxin A
• proteases

Question 8

PA biofilm makes it worse for the patient why?

Answer 8

Because it requires flagella type IV ili and over production of alginate.

BIO FILm is thick and makes it resistant to antibiotics and phagocytosis

Question 9

How does PAs alginate interacts?

Answer 9

Results in mucoid phenotype and may provide anti phagocytic functions.

Question 10

What is the manifestations of exotoxin A? Why is it so shitty?

Answer 10

COz its potent inhibitor of ADP ribosylation of elongation factor 2

SO IT KILLS EPITHELIAL CELLS VIA APEPTOTIC or non apeptotic mechanisms.

Question 11

Pesduomons Areginosa has many different antibiotic resistances

Answer 11

ITS A BITCH TO GET RID OFF.