Lecture 4: Protein techniques and organelles

Question 1

What is acetylation important for?

Answer 1

can regulate non-histone, protein stability, activity, and metabolism

Question 2

Why is methylation important

Answer 2

It can do the same things as acetylation along with cell signaling, localization and interactions

Question 3

What are the three major factors that influence protein function

Answer 3

1. Co-enzymes
2. Compartmentalization
3. Scaffolds

Question 4

What is the role of scaffolds in protein function

Answer 4

• They can bind multiple proteins to improve the probability of their interactions
• Can confine regions of proteins (condense/restrict)
• Basically just brings things together so that thigns like co-enzymes can react with the protein

Question 5

What is forster resonance energy transfer

Answer 5

When donor and acceptor fluorescent molecules allow for visualisation of protein interactions

Question 6

Why is fluorescence recovery after photobleaching important

Answer 6

allows us to study protein mvmt