Lecture 4 - Protein crystallization

Question 1

Standard protein concentration for crystallization

Answer 1

10 mg/mL

Question 2

The hanging drop method typically uses a ______ protein:well solution ratio

Answer 2

1:1

Question 3

As vapor returns to the well solution, the crystallization solution is concentrated towards

Answer 3

supersaturation (crystal nucleation)

Question 4

3 screening techniques for membrane proteins

Answer 4

1. Detergents
2. Bicelles
3. Lipidic Cubic Phase

Question 5

_____ crystals diffract to high resolution

Answer 5

Well ordered

Question 6

Crystals are formed by a sparse network of

Answer 6

weak intermolecular interactions

Question 7

Solubility diagrams are derived from

Answer 7

experimental data

Question 8

Crystallization is driven by

Answer 8

entropy

Question 9

To achieve crystallization, nucleation must overcome the kinetic barrier for phase separation from the

Answer 9

metastable solution

Question 10

From the metastable solution, it is equally likely that a nucleus will

Answer 10

dissolve back to the single phase, or grow into a crystal

Question 11

Higher supersaturation leads to formation of

Answer 11

stable crystallization nuclei

Question 12

Additives, detergents, and cofactors can often form

Answer 12

intermolecular contacts mediating crystal formation

Question 13

Does a direct correlation exist between crystallization pH and the pI of the protein?

Answer 13

No

Question 14

What are a few options that can be used to determine whether a crystal is a protein or a salt?

Answer 14

Dyes, GFP tags, UV

Question 15

On average, protein crystals contain about _____ percent solvent by volume

Answer 15

50