Lecture 3A Flashcards
DNA STRUCTURE & FUNCTION
JAMES WATSON & FRANCIS CRICK
Both: Double helix structure of the DNA
Both: Double helix structure of the DNA
JAMES WATSON & FRANCIS CRICK
CRICK
Central Dogma and Adaptor hypothesis
Central Dogma and Adaptor hypothesis
CRICK
WATSON
Erwin Schrodinger’s book “what is life”
Erwin Schrodinger’s book “what is life”
WATSON
GENE
a unit of heredity
a unit of heredity
GENE
GENOME
the entire set of genes in an organism; totality of genetic component
the entire set of genes in an organism; totality of genetic component
GENOME
LOCUS (LOCI)
a fixed location on a strand of DNA where a gene or one of its alleles is located
a fixed location on a strand of DNA where a gene or one of its alleles is located
LOCUS (LOCI)
ALLELES
two forms of a gene that occupy the same position on homologous chromosomes and that cover the same trait
two forms of a gene that occupy the same position on homologous chromosomes and that cover the same trait
ALLELES
DEOXYRIBONUCLEIC ACID (DNA)
material basis of heredity
material basis of heredity
DEOXYRIBONUCLEIC ACID (DNA)
biological macromolecule for the storage of genetic information
DEOXYRIBONUCLEIC ACID (DNA)
DEOXYRIBONUCLEIC ACID (DNA)
biological macromolecule for the storage of genetic information
universal for all bacteria, higher organisms
DEOXYRIBONUCLEIC ACID (DNA)
DEOXYRIBONUCLEIC ACID (DNA)
universal for all bacteria, higher organisms
DNA can be found in
– Nucleus (biparental inheritance)
– Plastids (uniparental inheritance)
* Mitochondria: maternal
* Chloroplasts:
> Angiosperms usually maternal
> Gymnosperms (conifers!) usually paternal
– Nucleus (biparental inheritance)
– Plastids (uniparental inheritance)
* Mitochondria: maternal
* Chloroplasts:
> Angiosperms usually maternal
> Gymnosperms (conifers!) usually paternal
DNA can be found in
Each nucleotide consists of:
- 5-carbon sugar (deoxyribose),
- nitrogen containing base attached to the sugar,
- phosphate group.
A forms…
2 hydrogen bonds to T,
G forms
3 hydrogen bonds to C
PURINE BASES (A and G)
larger of the two types of bases found in DNA
larger of the two types of bases found in DNA
PURINE BASES (A and G)
PYRIMIDINES (C and T)
The 6 atoms (4 carbon, 2 nitrogen) are numbered 1-6. Like purines, all pyrimidine ring atoms lie in the same plane
The 6 atoms (4 carbon, 2 nitrogen) are numbered 1-6. Like purines, all pyrimidine ring atoms lie in the same plane
PYRIMIDINES (C and T)
RIBOSE
a sugar, like glucose, but with only five carbon atoms in its molecule
a sugar, like glucose, but with only five carbon atoms in its molecule
RIBOSE
DEOXYRIBOSE
almost the same but lacks one oxygen atom
almost the same but lacks one oxygen atom
DEOXYRIBOSE
The deoxyribose, the phosphate and one of the bases combine to form a nucleotide
NUCLEOTIDES
NUCLEOTIDES
The deoxyribose, the phosphate and one of the bases combine to form a
DOUBLE HELIX
bases and sugar-phosphate chain
bases and sugar-phosphate chain
DOUBLE HELIX
characteristics of DNA (DNA vs RNA)
Double stranded
A pairs with T
G pairs with C
Resides in nucleus
Double stranded
DNA
A pairs with T
DNA
Resides in nucleus
DNA
G pairs with C
DNA
characteristics of RNA (DNA vs RNA)
Single stranded
A pairs with U
G pairs with C
Resides in nucleus and cytoplasm
DNA OF THE NUCLEUS
- Can be measured in base pairs since it is a double stranded molecule
- Higher organisms: 108 to 1011 base pairs
- Plants: particularly variable genome size
> Conifers: large - Repetitive sequences (102 to 108)
- Many sequences without known function
- Note: Genome of complex organism is not necessarily
larger than simple organisms
DNA OF PLASTIDS
Chloroplast DNA: cpDNA
Mitochondrial DNA: mtDNA
Chloroplast DNA: cpDNA
Approximately 120,000 – 200,000 bps
Approximately 120,000 – 200,000 bps
Chloroplast DNA: cpDNA
Completely sequenced for several plants
Chloroplast DNA: cpDNA
Chloroplast DNA: cpDNA
Completely sequenced for several plants
Pinus thunbergia
Chloroplast DNA: cpDNA (example)
Chloroplast DNA: cpDNA (example)
Pinus thunbergia
Mitochondrial DNA: mtDNA
Approximately 16,000 bps (human)
Approximately 16,000 bps (human)
Mitochondrial DNA: mtDNA
Mitochondrial DNA: mtDNA
Sequenced for human, some animals
Sequenced for human, some animals
Mitochondrial DNA: mtDNA