Lecture 35 - Pathophysiology of Multiple Sclerosis Flashcards
Multiple sclerosis
an immune-mediated (inflammatory) disorder involving destruction of the myelin sheath that surrounds neuronal axons
sclerosis: refers to scars
(sclerae, also known as plaques or lesions) that accumulate in the white matter of MS patients
MS etiology
a potential role for viral infections
Viral or bacterial infections may increase the risk of MS by activating autoreactive immune cells, leading to an autoimmune response in genetically susceptible individuals.
Evidence in support of this mechanism:
→ increased IgG synthesis in the CNS of MS patients
→ increased antibody titers to certain viruses
→ epidemiological data suggesting that childhood infection increases MS risk
Epstein-Barr Virus (EBV) may be involved in developing MS:
→ sequence similarities between EBV and self- peptides result in activation of autoreactive T- or B-cells (molecular mimicry).
→ increased antibody titers to Epstein-Barr nuclear antigen (EBNA) in MS patients. (EBNA mimics myelin basic protein that’s endogenous to us, autoimmine response leads to destruction of myelin sheath)
→ individuals with a particular HLA phenotype have an increased risk of developing MS when they also have anti-EBNA antibodies (this illustrates gene-environment interactions).
Different clinical forms of MS
Clinical forms of MS differ in terms of patterns of symptoms and intensities of inflammatory response:
relapsing-remitting MS (RRMS)
secondary progressive MS (SPMS)
primary progressive MS (PPMS)
clinically isolated syndrome (CIS)
Relapsing-remitting MS (RRMS): ~85% of cases
→ involves relapses of neurological dysfunction lasting weeks or
months and affecting the brain, optic nerves and/or spinal cord.
→ multifocal areas of damage are revealed by magnetic resonance
imaging, generally (but not always) in the white matter.
→ initial symptoms disappear, but less remission with each relapse
→ most cases of RRMS eventually enter a phase of SPMS.
Secondary progressive MS (SPMS)
→ characterized by less inflammation than RRMS
→ involves slowly progressive neurological decline and CNS
damage, with little remission.
Primary progressive MS (PPMS): ~15% of cases
→ resembles SPMS at the initial stage of the disease.
→ mean age of onset is later than RRMS (40 years vs. 30 years), perhaps because inflammatory episodes of RRMS surpass
the symptomatic threshold.
Clinically isolated syndrome (CIS)
→ an initial episode of neurologic symptoms lasting ≥ 24 h
→ involves inflammation and demyelination in the optic nerve,
cerebrum, cerebellum, brainstem or spinal cord (one or more foci)
→ most cases progress to MS
Progressive phase involves
cytodegeneration (loss of myelin, axons, oligodendrocytes) and occurs with a similar rate in the different forms of MS
The overall clinical presentation is determined by
the combination of the underlying degeneration (uniform, progressive) and the host’s immune reaction to it (intermittent, variable)
MS consists of what phases
autoimmune and degenerative
It is unclear which of the two phases is the disease trigger
Autoimmune phase
→ antigens released from the CNS or cross-reactive foreign antigens are presented to B and T cells in the lymph nodes.
→ B and T cells with high-affinity receptors for these antigens are expanded and migrate to CNS sites where they re-encounter and are activated by their target ligands.
→ activated B and T cells then carry out immune functions (release of antibodies and cytokines, respectively) at the CNS sites.
Degenerative phase
→ CNS damage is triggered by activated B and T cells or by other insults such as infection, or stroke.
→ antigens released from damaged sites in the CNS further prime immune cells in the periphery, thus completing a vicious cycle.
Autoimmune responses in MS
- dendritic cells that present CNS antigens (e.g. myelin basic protein) activate T-cell responses in the peripheral lymphoid tissue.
- activated B and T cells proliferate and infiltrate the CNS (this involves α4-integrin-mediated binding and penetration of the BBB).
- after re-encountering their specific antigen in the CNS, B cells mature to plasma cells and release IgG antibodies that target the antigen on expressing cells
- T cells interact with their target ligands presented by oligodendrocytes, neurons, or microglia on MHC molecules.
- T cell activation results in cytokine release and macrophage stimulation, leading to damage to the myelin sheath.
A closer look at autoimmune responses in MS
- CD8+ T cells engage oligodendrocytes via T-cell receptor/MHC class I interactions.
- cytokines released by T cells include IFN-γ, TNF-α, perforin, and granzyme (leading to oligodendrocyte destruction).
- antibodies trigger the activation of complement (C5-9 membrane complex) on oligodendrocyte membranes, resulting in pore formation and cell damage.
- macrophages (Mφ) recruited to the inflammatory lesion release toxic agents (e.g. reactive oxygen and nitrogen species; glutamate) that harm oligodendrocytes.
- macrophages also harm the myelin sheath via phagocytosis.