Lecture 3: Microbial Cell Structure/Function Part 2 Flashcards

1
Q

What are pili?

A

Thin protein appendages on the outside of the cell that help with attachment and can facilitate horizontal gene transfer

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2
Q
  • What genus is this?
  • What life domain does it belong to?
  • What type of environement does it live in?
A
  • Haloquadratum
  • Archaea
  • High salt environement
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3
Q

What type of bacteria is this and why (2 reasons)?

A

Gran negative bacteria because there is an outer membrane and a THIN layer of peptidioglycan

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4
Q

What kind of genetic material do archaea have?

A

Have circular, double stranded DNA chromosomes as well as plasmids

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5
Q

What is methanogenesis?

What doman of life is it unique to?***

A

Biological production of methane gas (very potent greenhouse gas)

Unique to archaea

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6
Q

What happens to the motion of a cell as the concentration of attractant increases?

A
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7
Q

What is this flagellation pattern?

A

Polar (1 end of the cell) monotrichous

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8
Q
  • What organism is this?
  • What life domain is it from?
  • It is a source of _____ for ______
A
  • Pyrociccus furiosus aka “rushing fireball”
    • one of Dr. Mohr’s favorite organisms
    • Hyperthermophile (85-113 degrees)
      • Can live in extremem environement due to the archaea membrane
    • Has more than 50 flagella
  • Archaea
  • Source of Pfu polymerase for PCR
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9
Q

Do Archaea have plasma membranes?

****

A

Yes, archaea have plasma membranes, aka lipid bilayer, cytoplasmic membrane

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10
Q

What type of motion do repellents cause?

What’s that motion called?

A

Cause clockwise motion

Called tumble

The flagella fly apart, cells then tumble and change their direction

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11
Q

What flagellation pattern is this?

A

Peritrichous—-flagella spread out all over the entire surface

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12
Q

What is the collective pattern of runs + tumbles called?

A

Random walk

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13
Q

What flagellation pattern is this?

A

Lophotrichous: cluster of flagella at one or both ends

This is specifically polar lophotrichous because flagella only at one end of the cell

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14
Q

What temperature range do hyperthermophiles live in?

A

85-113+ degrees C

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15
Q

How does the size of most archaea compare to bacteria?

A

Similar sized to bacteria, in the um (micrometer) range

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16
Q

What are Type IV Pili?

A

Dynamic (can move) that can extend and retract, which is called

“twitching motility”

17
Q

Where do archaea live?

A

Live in terrestrial and aquatic environements

18
Q

What are the areas labelled called?

A
  1. Filament
    1. Made up from flagellen protein
  2. Hook
    1. Connects basal body to the filament
  3. Basal body
    1. Series of rods and circular rings/discs that make up the motor
  4. H+ protons moving into the cell to provide energy for the basal body (proton gradient)
19
Q

What are functions of external structures on microbes?

A
  • Attachment
  • Horizontal gene transfer (via sex pili)
  • Movement (flagella)
20
Q

What kind of motion do attractants cause? (CW or CCW?)

What direction is that motion in?

What is it called?

A

Cause counterclockwise motion.

Flagella bundle together

Cells “run” forward

21
Q

How do archaea reproduce?

A

Asexually

22
Q

What are the proteins in the cell membrane?

What do they do/perform?

A

Chemoreceptors

They detect componds in the external environment and aid in chemotaxis (sensory system that enables microbes to move towards or away from specific chemicals)

23
Q

What temperature range do thermophiles live in?

A

Live in 45-85 degree C temperatures

24
Q

Do archaea have cell walls?

****

A

Some archaea have cells walls, but DO NOT HAVE petidoglycan (NAG-NAM).

Other archaea don’t have cell walls but have a protein based S-layer