Lecture 3: Basics of cell science Flashcards

1
Q

What are the basic components of all cells?

A
  • Plasma membrane
  • Cytosol
  • Ribosomes
  • Chromosomes
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2
Q

What are the three fundamental domains of life:

A
  • Bacteria ( prokaryote)
  • Archaea (prokaryote)
  • Eukarya (eukaryote)
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3
Q

What are the eukaryote kingdoms?

A
  • Animalia
  • Plantae
  • Fungi
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4
Q

What is the spontaneous germ theory?

A

That micro organisms spontaneously appear from non-living organisms.

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5
Q

Define the term resolution:

A

The minimum distance that can separate two points that still remain identifiable as separate points when viewed under a microscope

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6
Q

Define the term magnification:

A

The ratio of an object’s size to its real size

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7
Q

What is the germ theory?

A

It disproved spontanous generation - microorganisms do not spontaneously appear. Theory provided evidence that diseases were caused by microorganisms within the body

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8
Q

What are Kosh’s postulates?

A
  1. Organims/agent must be found in every case of the disease
  2. Agent must be isolated & grown outside of the host
  3. Inoculate health host, host must get the disease
  4. Isolate agent in dead host - agent found in dead host
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9
Q

What magnification does the light microscope allow?

A

1000x

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10
Q

What magnification does the EM microscope allow?

A

100,000x

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11
Q

How does the light microscope work?

A

visible light passed through specimen then through lens

- lens refracts the light so image is magnified

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12
Q

How does the EM work?

A

Focuses a beam of electrons through a specimen or onto the surface.

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13
Q

What does the scanning electron microscope do?

A

Scans the outer surface of the sample.

  • 3D
  • black & white
  • dead specimen
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14
Q

What does the transmission electron microscope do?

A

Shows the internal structures of the specimen

  • 2D
  • black & white
  • dead specimen
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15
Q

What does the light microscope do?

A

Allow the observation of living tissue

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16
Q

What is the main advantage of small cell size?

A

a small cell has a large surface area relative to cytoplasmic volume