Lecture 27 - Beta Oxidation of Fatty Acids

Question 1

What are the stages of Beta oxidation of fatty acids

Answer 1

Activation
Transport
Oxidation

Question 2

What is in the activation stage

Answer 2

Attached to COENZYME A

To form –
Acyl-CoA

Occurs at the outer surface of the mitochondria
Catalysed by Acyl-CoA synthetase

RCOO- + CoA + ATP <—> ACYL-CoA +AMP + PPi

Reaction is driven to the right by removal of a product

Question 3

Where is the removed product from activation transported to

Answer 3

Mitochodria

Question 4

What occurs in the transport section

Answer 4

Acyl group becomes attached in place of hydroxyl and releases Coenzyme A

Question 5

What occurs in the oxidation phase

Answer 5

2 carbon unit is removed by a four step process

Process repeats if fatty acid is still 4 or more carbons long

(slide 16)

Question 6

what are the 4 steps in the oxidation phase breaking down eg. a 16 carbon fatty acid

Answer 6

Oxidation
Hydration
Oxidation
Thiolytic cleavage
(Slide 17-20)