Lecture 22: DNA Replication Flashcards
1
Q
What are DNA polymerase requirements?
A
Requires:
- all four deoxyribonucleoside triphosphates (dATP, dGTP, dCTP, dTTP)
- magnesium ions Mg 2+
- a primer (sequence of bases used to initiate synthesis synthesised by primase). Growing chain only occurs by attaching deoxyribonucleoside mono phosphate to the 3’ OH end of DNA
- a template strand
2
Q
DNA polymerase 3 is essential for prokaryotic DNA replication
A
-only DNA polymerase 3 works fast enough to account for the rate of DNA replication in cells
3
Q
DNA replication by DNA polymerase
A
DNA polymerase synthesize exclusively in the 5’ to 3’ direction
-sands are anti parallel
4
Q
Much much more in this lecture that is boring as bat shit so need to go over it
A
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