Lecture 21 - Methods of protein analysis II Flashcards
1
Q
Acid hydrolysis is a method to determine
A
the amino acid composition of a protein
2
Q
Edman degradation is used to
A
determine the amino acid sequence
3
Q
Edman degradation sequentially
A
removes one residue at a time from N-terminal of peptide
4
Q
Enzymatic fragmentation is used because
A
it can be difficult to measure large proteins
5
Q
Trypsin cleaves
A
on the C-terminal side of lysine and arginine
6
Q
Cyanogen bromide acts only on
A
methionine residues
7
Q
Mass spectrometry of peptide fragments is an alternative to
A
Edman degradation
