Lecture 20 Flashcards

1
Q

Explain dimerization domains (2 parts)

A

The basic region contacts the DNA.

The Leu-zipper acts as the dimerization on the hydrophobic interface.

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2
Q

Homodimers

A

2 proteins are the same

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3
Q

Heterodimers

A

2 proteins are different

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4
Q

Steroid receptor

A

Activation domain. Once the ligaand binds, tx factors are activated, and the structure change occurs.

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5
Q

bHLH

A

basic protein HELIX-LOOP-HELIX

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6
Q

What happens when 2 bHLHs bind?

A

dimerization occurs and the basic region binds to DNA

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7
Q

What happens when a nonbasic HLH and bHLH bind?

A

the heterodimer can’t bind to the DNA

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8
Q

What is required for RNA pol II to initiate transcription?

A

TFII X

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9
Q

what sequence is at the initiator of POL II

A

Py(2) CA Py(5)

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10
Q

Py’s are

A

pyrimidines

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11
Q

Inr

A

Initiator

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12
Q

DPE

A

downstream promoter element

in promoters w/o TATAA box

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13
Q

2 types of core promoters

A
  1. TATA

2. TATA less

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14
Q

What does the core promoter for RNA pol II have

A

INR region

either a tATa or DPe

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15
Q

TFIID

A

TBP (tata bind proteins)

TAFS (TBP associated factors)

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16
Q

TBP

A

tata binding protein

17
Q

TAFS

A

TBP associated factors

18
Q

Where does TBP bind?

A

To the TATA box in the minor groove

19
Q

What happens when TBP binds?

A

The DNA bends giving more exposure

20
Q

Step 1 of pol II

A

TBP binds to TAFS to form TFIID

21
Q

Step 2 of pol II

A

TF2 F and TFII B bind

22
Q

Step 3 of pol II

A

TFII E comes in

23
Q

Step 4 of pol II

A

TF II H comes in with kinase activity and puts - charges on carboxy terminal domain

24
Q

Step 5 of pol II

A

Repulsion event causes transcription to begin and RNA pol II takes off

25
What are required to melt DNA to allow the polymerase to move?
TF II E and H
26
Explain the cycle of phosphorylation
1. Recruitment of RNA pol II 2. Phosphorylation recruits enzymes to cap 5' end 3. Elongation occurs 4. Dephosphorylate and cycle continues
27
What does the C terminal domian have that can be phoshorylated/
Threonine Tyrosine Serine
28
Carboxy Terminal Domain
coordinates processing and export of tRNA from nucleus
29
Which promoters do enhancers activate?
the ones nearest itself
30
UAS
upstream activating sequence found in yeast | behaves like an enhancer but only works upstream
31
What happens if the two molecules are catenated?
Transcription can occur! Enhancers can be made to work in trans as long as it's constrained to be in proximity of the promoter.