Book Vocab Flashcards
Origin for replication in yeast. Common feature is a conserved 11bp sequence called the A domain.
ARS
Origin recognition complex,a multiprotein complex found in eukaryotes that binds to the replication origin and remains associated with it throughout the cell cycle
ORC
Pre-replication complex
a protein-DNA complex at the origin in S. cerevisia that is requried for DNA replication. it contains the ORC complex, CDC6, and the MCM proteins
Post replication complex
A protein-DNA complex in S. cerevisia that consists of teh OrC complex bound to the origin.
Recombination involving reciprocal exchange of sequences of DNA, between two chromosomes that carry the same genetic loci
Homologous recombination
Recombination that occurs between two specific sequences, as in phage integration/excision or resolution of cointegrated structures during transposition
Site-specific recombination
Recombination that occurs in non-germ cells. It doesn’t occur during meosis.
Somatic recombination
Hotspot
A site in the genome at which the frequency of a mutation is very much increased
A pair of DNA duplexes that are connected together through a reciprocal exchange of genetic material
Joint molecule
The loop of displaced DNA generated by strand invasion and extension during homologous recombination
D-loop
Breaks that occur when boths trands of a DNA duplex are cleaved at the same site. It iniates genetic recombination
Double strand breaks
Axial element
a proteinaceous structure around which the chromosomes condense at the start of synapsis
Lateral elements
when chromosomes become aligned and the synaptonemal complex forms the axial elements become called lateral elements
Central element
Structure that lies in the middle of the synaptonemal complex. It’s formed from ZIP proteins.
Group of proteins that’s equivalent to the lateral element, and forms an axis for each pair of sister chromatids to extend
Cohesins
Group of proteins equivalent to the central element, that connect lateral elements with transverse filaments
Zip proteins
What do mutations in cohesin do?
double strand breaks can still occur but they Block the formation of recombinants
What do mutations in the Zip proteins do?
the lateral elements can form and become aligned, but they do not become closely synapsed.
Recombination nodules
Dense objects present on the synaptonemal complex that may represent protein complexes involved in crossing over
Closed complex
stage of initiation of transcription where the polymerase hasn’t caused the two stands to separate yet. The DNA is double stranded.
Open complex
stage of initiation of transcription where RNA polymerase causes the two strands of the DNA to separate to form the transcription bubble
Tight binding
binding of RNA polymerase to DNA in formation of an open complex
Ternary complex
complex in initation that consists of RNA polymerae and DNA as well as dinucleotide that represents the first two bases in the RNA product
Abortive initation
process in which RNA polymerase starts transctiption but terminates before it has left the promoter.
