Lecture 2: Cells Flashcards
what are the two main types of cells
Prokaryotic and Eukaryotic
where are prokaryote cells found
found in 2 groups of single-celled microorganisms (Bacteria and Archaea)
where are eukaryotic cells found
plants and animals
magnification
size of image / size of specimen
size of image
size of specimen / magnification
size of specimen
size of image / magnification
resolution
Resolution is how detailed the image is. Specifically, it is how well a microscope distinguishes between two points that are close together
cm to mm
x10
mm to um
x1000
um to nm
x1000
nm to um
/1000
um to mm
/1000
mm to cm
/10
stage
microscope slide is placed here
condenser
used to vary the intensity of light reaching the object
fine focus
used to focus the high power objective lence
coarse focus
used to focus the low and medium power objective lenses
objective lenses
x4 (low), x10 (medium), x40 (high power)
turret
rotates to bring the objective lenses into place
magnification =
magnification = size of image / size of specimen
prokaryotes
- smaller than eukaryotes
- oldest
- Single-celled organisms
- Simple structure
- Don’t have membrane bound organelles in - the cytoplasm
Binary fission
No nucleus
Single shorter circular DNA
Nucleoid
Smaller ribosomes-70S (not attached to ER)
Cell wall contains murein (glycoprotein)
Plasmids
Capsule
flagella
binary fission
produce an identical copy (genetically identical)
- Replication of circular DNA and plasmids
- Division of cytoplasm to produce 2 daughter cells each with a single copy of the circular DNA and variable number of copies of plasmids
DNA prokaryotes
- Prokaryotes carry DNA as chromosomes
- Shorter and circular
- DNA isn’t wound around histones
- It condenses to fit in the cell by supercoiling
DNA eukaryotes
- Eukaryotic cells contain linear DNA molecules
- Exist as chromosomes (thread like structures)
- DNA – long (wound up to fit in the nucleus)
- Wound around proteins called histones (help to support the DNA)
- DNA (and protein) coiled up very tightly
- Mitochondria and chloroplasts have their own DNA similar to prokaryotic DNA
- Circular and shorter
- Not associated with histone proteins