Lecture 2

Question 1

How many lens does a simple microscope have?

Answer 1

1

Question 2

objective lens x ocular lens =

Answer 2

total magnification

Question 3

the ability of the lens to distinguish 2 points

Answer 3

resolution

Question 4

what is the resolution of the best light microscope

Answer 4

0.2 micron

Question 5

Shorter wavelengths of light provide

Answer 5

greater resolution

Question 6

measure of the light bending ability of a medium

Answer 6

refractive index

Question 7

What is used to keep light from bending?

Answer 7

immersion oil

Question 8

What kind of background are dark objects visible against

Answer 8

bright ones

Question 9

What backgrounds are light objects visible against?

Answer 9

dark ones

Question 10

Accentuates diffraction of the light that passes through a specimen

Answer 10

phase-contrast microscopy

Question 11

Accentuates diffraction of the light that passes through a specimen; uses two beams of light

Answer 11

differential interference contrast microscopy

Question 12

Absorb UV light and emit visible light

Answer 12

Fluorescence microscopy

Question 13

popularly used as a tag to illuminate living cells in a florescent microscope

Answer 13

GFP

Question 14

Uses electrons instead of light

Answer 14

Electron microscopy

Question 15

Produces a three-dimensional image

Answer 15

Confocal microscopy

Question 16

Specimens may be stained with heavy metal sales

Answer 16

Transmission electron microscopy (TEM0

Question 17

Ultrathin sections of specimen. The light passes through the specimen, then an electromagnetic lens to a screen or film

Answer 17

TEM

Question 18

Produces a beam of electrons that scans the surface of a whole specimen.

Answer 18

Scanning electron microscopy (SEM)

Question 19

Secondary electrons emitted from the specimen produce the image

Answer 19

SEM

Question 20

Uses a metal and diamond probe inserted into the specimen and produces three dimensional image

Answer 20

Scanned probe- Atomic force microscopy

Question 21

Coloring the microbe with a dye that emphasizes certain structures

Answer 21

staining

Question 22

A thin film of solution of microbes on a slide

Answer 22

smear

Question 23

the chromophore is a cation

Answer 23

in a basic dye

Question 24

the chromophore is an anion

Answer 24

An acidic dye

Question 25

staining the background instead of cells

Answer 25

negative staining

Question 26

use of simple basic dye

Answer 26

simple stain