Lecture 18: Molecular Bio in Medicine

Question 1

Molecular biology has and will improve

Answer 1

human health

Question 2

Molecular cloning is

Question 3

Molecular cloning purposes

Question 4

To obtain DNA of interest, one can use?

Question 5

Polymerase Chain Reaction (PCR)

Question 6

PCR amplifies?

Question 7

Three steps of PCR

Question 8

Advantages of PCR

Question 9

Application of PCR

Question 10

RT-PCR used to obtain?

Question 11

PT-PCR enzyme

Question 12

RT-PCR uses

Question 13

RT-PCR protocol

Question 14

RT-PCR can amplify…

Question 15

Manipulate DNA for cloning uses

Question 16

Restriction endonucleases (or restriction enzymes) function

Question 17

Restriction enzymes

Question 18

“Sticky” ends vs. blunt ends

Question 19

Palindrome in dsDNA

Question 20

Restriction enzymes can leave?

Question 21

After PCR and endonucleases…

Question 22

Gel electrophoresis

Question 23

GE, molecules separate based on

Linear DNA separates by….

Question 24

DNA staining with fluorescent dyes steps (4)

Question 25

Polymorphism detection using?

Question 26

RFLPs & example

Question 27

Detection of RFLPs by PCR

Question 28

DNA needs to be placed in

Question 29

Plasmid/Vector

Question 30

Plasmid/Vector uses

Question 31

Plasmid DNA (cloning vector) contains three elements?

Question 32

Molecular cloning

Question 33

How to place target DNA into plasmid (2)

Question 34

Making recombinant DNA main steps

Question 35

B-lactamase

Question 36

DNA Ligase in recombinant DNA (3 steps + note)

Question 37

DNA sequencing: determine the exact order of nucleotides requires? What method?

Question 37

To confirm the correct clone, what must happen?

Question 38

dNTP

Question 39

ddNTP

Question 40

Dideoxynucleotide ddNTP function

Question 41

DNA polymerase catalyzes?

Question 42

Addition of a nucleotide mediated through 3’-OH group?

Question 43

Incorporated dideoxynucleotide lack a?

Question 44

Sequencing reaction mix contains all reagents including?

Question 45

Sequencing protocol steps (4)

Question 46

Each synthesized DNA has specific length

Question 47

DNA length matched to

Question 48

Fluorophore steps

Question 49

Fluorescent color = ?, Migration in gel = ?