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Biochem > Lecture 15: Translation > Flashcards

Lecture 15: Translation Flashcards

1
Q

Once an mRNA has been produced by transcription process, the information
present in its nucleotide sequence is used to

A

synthesize a protein.

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2
Q

Translation is a process of converting nucleic acid codes into

A

amino acid sequences

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3
Q

Coding sequences (open reading frame) is the

A

translated region, while the untranslated regions are used for controlling translation and mRNA stability

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4
Q

Translation of an mRNA molecule begins at the 5’ end of the

A

mRNA and proceeds in the 3’ direction.

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5
Q

Polypeptide synthesis begins at the

A

amino terminus (“NH2)

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6
Q

The sequence of nucleotides in the linear mRNA molecule is read consecutively in groups of

A

Three. It is read from a fixed starting point: AUGGGGCUCAGCGAC is read
as AUG-GGG-CUC-AGC-GAC–.

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7
Q

Codon

A

Each group of three consecutive nucleotides in RNA. Each
codon specifies either one amino acid or a ‘stop’ codon to the translation process.

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8
Q

genetic code

A

The nucleotide sequence of a gene is translated into the amino acid sequence of a protein by rules known as the genetic code. Genetic code is non-overlapping and continuous.

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9
Q

All proteins (ORFs) start with an

A

AUG codon, which codes for methionine. In prokaryotes, it is a modified methionine called “formyl-methionine.”

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10
Q

Translated is halted at

A

stop codons

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11
Q

3 nucleotides specificy

A

1 amino acid (codon)

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12
Q

How to read codons

A
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13
Q

Three stop codons

A

UAA, UGA, UAG (you are away, you go away, you are gone)

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14
Q

Specificity of genetic code

A

a particular codon always codes for the same amino acid
for example: UUA will always code for leucine

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15
Q

Universality of genetic code

A

its specificity has been conserved from very early stages of evolution

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16
Q

Degeneracy (redundancy) of genetic code

A

several codons can be used to code for the same AA. For example, leucine is specified by six different codons.

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17
Q

Nonsense mutation

A

base substitution results in the introduction of a stop (termination) codon (changing a single nucleotide base in the coding region of mRNA)

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18
Q

Silent mutation

A

base substitution does not change the identity of the incorporated AA (changing a single nucleotide base in the coding region of mRNA)

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19
Q

Missense mutation

A

base substitution results in an AA change (changing a single nucleotide base in the coding region of mRNA)

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20
Q

The sequence of nucleotides is read consecutively in groups of

A

three

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21
Q

insertion or deletion of “N” number of bases where “N” is not evenly divisible by 3 alters

A

the reading frame of the rest of the translated mRNA. This is called a frameshift mutation. This can induce missense and nonsense mutation.

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21
Q

Occasionally, a sequence of three bases that is repeated in tandem will become amplified in number so that

A

many copies of the triplet occur. If this happens within the coding region of a gene, the protein will contain many extra copies of one AA.

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22
Q

expansion of CAG codon (glutamine) in

A

exon 1 for huntington protein. Example of tri-nucleotide repeat extension.

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23
Q

The expansion in the coding region results in
abnormally long protein - when cleaved, it produces

A

toxic fragments that aggregates in neurons, causing
neurodegenerative Huntington disease

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24
Q

Expansion of CGG repeats (arginine)

A

fragile X syndrome

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25
Q

The abnormal expansion prevents
production of (Fragile X)

A

FMRP. This loss disrupts nervous system function, leading to signs of fragile X syndrome, the common cause of intellectual disability seen in males.

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26
Q

Expansion of CUG (serine) is seen in

A

myotonic dystrophy

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27
Q

3 stages of translation

A

initiation, elongation, termination

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28
Q

mRNAs

A

-made by RNA polyII
-template

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29
Q

tRNAs

A

-made by RNApolyIII
-decodes triple codonsAm

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30
Q

Aminoacyl-tRNA synthesase

A

attach appropriate amino acids to tRNAs with specific anticodons

31
Q

Ribosomes

A

site of translation

32
Q

GTP

A

GTP is used as the energy requiring steps in translation

33
Q

ATP

A

used to charge tRNAs

34
Q

tRNA

A

decodes codons

35
Q

tRNA decodes by using

A

base pairing between the codon on the mRNA and the anti-codon on the tRNA

36
Q

Codon and anticodon pairing reflects

A

complementary (antiparallel) binding

37
Q

At least one specific type of tRNAs is required for

A

each amino acid (in humans, there are more than > 50 tRNAs)

38
Q

all tRNAs have

A

similar structures

39
Q

each tRNA has an attachment site for a specific _______ ______ at what end?

A

specific amino acid, at its 3’ end (AA is attached at the 3’-OH end of the A nucleotide in the -CCA sequence)

40
Q

An enzyme is required for attachment of AA to their corresponding

A

tRNA

41
Q

aminoacyl-tRNA synthetases are responsible for

A

adding correct AA to their appropriate tRNAs

42
Q

There are ____ different aminoacyl-tRNA synthesases

A

20, one for each AA. This specificity contributes to the high fidelity of the translation process.

43
Q

Aminoacyl-tRNA synthetase enzyme catalyzes a

A

two step reaction that results in the covalent attachment of an AA to the 3’ end of its corresponding tRNA

44
Q

1st step of aminoacyl-tRNA synthetase

A

coupling of AA to AMP to form aminoacyl-AMP

45
Q

2nd step of aminoacyl-tRNA synthetase

A

enzyme transfers AA from aminoacyl-AMP to tRNA to form aminoacyl-tRNA (aa-tRNA). aa-tRNA and AMP are released from the enzyme. The overall reaction requires ATP, which is cleaved to AMP and PPi (inorganic pyrophosphate).

46
Q

aminoacyl-tRNA synthetase has

A

proofreading and editing activity that can remove an incorrect AA from the enzyme or the tRNA molecule

47
Q

Aminoacyl-tRNA synthetase steps

A
  1. The AA and ATP bind to synthetase
  2. The AA is attached covalently to AMP, releasing PPi
  3. Coupling of AA to AMP to form aminoacyl-AMP
  4. The enzyme binds the appropriate tRNA, and the AA is transferred to the 3’ end of the tRNA. The tRNA is now said to be charged, “charged tRNA” has AA attached to it. An aminoacyl tRNA is a charged tRNA. This has a high energy bond that will help drive the peptide bond formation forward.
48
Q

Ribosomes are sites of

A

protein synthesis

49
Q

mRNAs are translated by

A

ribosomes

50
Q

Ribosomes are multi-subunit…

A

rRNA-containing protein complexes

51
Q

Two subunits

A

large and small

52
Q

Large subunit

A

catalyzes peptide bond formation.

52
Q

Small subunit

A

is a decoding center,
i.e., binds mRNA and determines the
accuracy by insuring correct base-pairing between codon and
anticodon.

53
Q

A ribosome has three tRNA binding sites

A
  1. A (aminoacyl) site: a charged tRNA is presented to the mRNA to be translated
  2. P (peptidyl) site: the growing peptide chain is attached as a peptidyl-tRNA complex
  3. E (exit) site: contains empty tRNAs

(Each of the sites extends over both subunits)

54
Q

In eukaryotic cells, the ribosomes are either

A

free in the cytosol or in association
with the rough endoplasmic reticulum (RER).

55
Q

After mRNA processing and splicing are complete, the mature mRNAs are

A

exported from the nucleus of the cell into the cytoplasm

56
Q

in the cytoplasm, polypeptides can be synthesized by

A

free ribosomes in the cytosol or by ribosomes bound to the rough endoplasmic reticulum (RER).

57
Q

Cytosolic ribosomes

A

synthesize proteins that are required in the cytosol itself or destined for the nucleus, mitochondria, or peroxisomes.

58
Q

RER-associated ribosomes

A

synthesize proteins that are to be exported from the cell, incorporated into membranes, or imported into lysosomes

59
Q

In eukaryotes, the transcription process must
end before the

A

translation process can start - described as “uncoupled transcription & translation”

60
Q

Prokaryotes have “coupled

A

transcription & translation” process.

61
Q

For both prokaryotic and eukaryotic, translation factor proteins are required for

A

translation, and these fall into one of three classifications:
-Initiation factors (IF)
-Elongation factors (EFs)
-Release factors (RFs)

62
Q

Eukaryotic translation factors are distinguished from prokaryotic by an

A

“e”
prefix (eIF1, eEF2, eRF1, etc.)

63
Q

Prokaryotes utilize

A

Shine-Dalgarno sequence for translation initiation.

64
Q

Shine Delgarno Sequence

A

sequences are located 6-10 bases upstream of the start codon (AUG) in the 5’UTR region of the mRNA transcript.

65
Q

The rRNA of the small ribosomal subunit (FYI, 16S) binds to

A

Shine-Dalgarno sequence and positions the small ribosomal subunit to promote efficient and accurate translation of mRNA.

Sequences 5’end of the mRNA and the 3’ end of the 16S rRNA form
complementary base pairs.

66
Q

The initiation tRNA (tRNAiMet)

A

the tRNA that carries the initiating methionine,
binds to the start codon.

67
Q

In prokaryotes, the initial methionine carries

A

N-formylated methionine (fMet)

68
Q

Translation Initiation in Prokaryotes

A
  1. Initiation factors (IFs) and GTP bind to the small ribosomal subunit
  2. mRNA assemble with the small ribosomal subunit (initiator tRNA recognizes the start codon and bind to the small ribosomal subunit. Binding to mRNA is positioned around the Shine-Delgarno sequence of mRNA.
  3. Large ribosomal subunit joins the small initiation complex.
  4. GTP is hydrolyzed and IFs are released.

Now, a functional ribosome is formed with a charged tRNAimet in the P site. The A site is empty.

tRNAiMet is always deposited into the P site.

69
Q

Translation initiation in eukaryotes occurs in the

A

nucleus, and translation occurs in the cytoplasm –? referred to as compartmentalization of processes

70
Q

Eukaryotes mRNA do not have

A

Shine-Delgarno sequences

71
Q

Once in the cytoplasm, 5’ cap of mRNA is bound by small

A

ribosomal subunit along with eIF4G and eIF4E. The subunit moves from the 5’ to 3’ direction along the mRNA until it encounters the initiator AUG.

72
Q

Proteins that bind to the 5’ cap associates with proteins that

A

bind to the poly-A-tail –> for efficiency of re-initiation of translation

73
Q

Translation initiation in eukaryotes (4 steps)

A
  1. Eukaryotic initiation begins with the charged initiator tRNA (tRNAiMet) binding to GTP bound eIF2
  2. eIF2/GTP/tRNA complex binds to the small ribosomal subunit
  3. Meanwhile, eIF4E/4G bind to the 5’ cap end of the mRNA
  4. The small ribosomal subunit complex binds to the mRNA/eIF4
74
Q
A

Biochem (7 decks)

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