Lecture 17: Canine Lymphoma

Question 1

What is a physical examination abnormality that you may find in a dog with Lymphoma (heterogeneous disease)?

Answer 1

LN: Generalized Peripheral Lymphadenopathy (massive)
- Mandibular
- Popliteal
- Prescapular

• Decreased appetite (hyporexia)
• Lethargy

Question 2

What is the Diagnostic Plan for Canine Lymphoma?

Answer 2

• Fine needle aspirate (Lymph node)
• Immunophenotyping (Flow cytometry)
• CBC
  
  • Anemia of chronic disease
  • Cytopenia
  • Atypical lymphocytes / unclassified cells
• Serum Chem
  
  • Hypercalcemia
• Profile
• U/A
  
  • Secondary UTI
• +/- Thoracic radiographs
• +/- Abdominal ultrasound
• +/- Bone marrow aspirate

Question 3

Term:
The process by which antigens in tissue sections are made visible by the use of fluorescent dye or enzyme markers

Answer 3

Immunohistochemistry

Question 4

Term:
- PCR for antigen receptor rearrangement
- Evaluation of clonality by PCR
- Canine assay is more sensitive than the feline
- Negative results do not rule out clonality - just means the assay did not detect clonality

Answer 4

PARR

Question 5

What do we stage?

Answer 5

Patient
- assessment
- prognostic factors
- treatment decisions

Question 6

What do we grade?

Answer 6

Tumor

Question 7

What are the goals of therapy?

Answer 7

• Quality of Life
• Toxicity
• Cost
• Convenience
• Survival vs. Remission

Question 8

List Treatment options:

Answer 8

Combination chemotherapy
- CHOP (12 months, gold standard)
Single-agent chemotherapy
- Doxorubicin (6-8 mo), Lomustine (CCNU), Rabacfosadine (Tanovea)
Prednisone (alone 1-2 months)