Lecture 16 T cell mediated immunity Flashcards
3 development of the immune response
- nonspecific
- Central (priming) phase
- T cell effector phase
types of professional antigen cells
Dendritic cells
macrophages
B cells
Which cells prime naive T cells
dendritic cells
TFH express _____ and help B cells
CXCR5
What is signal 3?
delivery of cytokines from DC to T cell. Drives differentiation into functional effector role;
match effector response to the specific problem
what are the main function of TH1 cells
activate macrophages, provide help with antibody production. They target microbes in intracellular vesicles and extracellular bacteria
What is the main function of TH2
they help B cells class switch to IgE. Target helminth parasites
What is main function of TH17
- get rid of fungal infections
- maintaining gut epithelial barrier
- enhance neutrophil response
- promote barrier integrity (homeostastis)
CTL kills ____
virus infected cells (and cancer)
TFH main function
target all kinds of pathogens
help B cells with antibody production, isotype switching
CD4 regulatory cells function to
suppress T cell response
Which cytokinesspecify TH1 through T-bet?
IFN-gamma and IL-12 through STAT 1 and STAT4
what cytokines specify TH2 though GATA-3?
IL4 through STAT6
which cytokines specify TH17 through RORgammaT?
TGFbeta, IL23, IL6 though STAT3
Which cytokines specify TFH through Bcl-6?
IL6, TGF-beta, IL-21 through STAT3
Which cytokines specify Treg through FoxP3?
IL2, TGF-beta, IL10 through STAT5
what cytokines do TH1 cells make
IFN-gamma
what cytokines do TH2 cells make
IL4, IL5, IL13
What cytokines do TH17 cells make
IL17, Il22
What cytokines do TFH cells make?
IL21
What cytokines do iTreg cells make
TGF-beta, IL10
IFN-gamma and CD40L produced by ____ drive fusion of intracellular compartments in ______
TH1; macrophages
IL4 activates _____, IL5 activates _______, IL13 helps to enhance barrier mucosal immunity, all produced by ___
mast cells and basoophils;
eosinphils;
TH2
IL17 affects ____ cells and IL22 affects ____ cells with which will activate them to recruit _______ to the site of infection. The production of these cytokines is mediated by _____.
stromal cells;
epithelial cells;
neutrophils;
TH17
____ produce Il21 that targets B cells and promotes expression of ______ to determine the B cell thats made
TFH; CXCR5
____ cells inhibit DCs though expression of IL10, TGF-beta, and IL35
Treg
Leishmaniasis is caused by _____ that infect macrophages and dendritic cells. Using susceptible/resistance mice strains and watching the response shows the T cell response that determines resistance. ___ is shown to play a role
sand fly protozoans. TH17
BALB/c mice are ______ to Leishmania major because they generate ____ mediated ____ response.
susceptible, IL4, TH2
C57/Bl6 are _____ because they generate ______ mediated ____ response
resistant; IFN-gamma; TH2
BALB/c mice treated with ______, that reverses ____ response
an antibody that blocks IL-4;
TH2/Th1
Mycobacterium leprae has a _______ leprosy with TH1 and _______ leprosy with TH2 responses
Th1, Th2
IL4 secreted by ___ can inhibit differentiation of _____ cells because they block ____ receptor. Downregulation of STAT4 mediated signaling of this cytokine also decreases ___ production, inhibiting production of ______
TH2,
TH1, IL12,
IL23, Th17
IFN-gamma produced by ___ cells leads to downregulation of ____ and the inhibition of ___ cells and downregulation of ____
TH1, IL4rec, TH2, IgE rec
Treg cells can inhibit the production of ___ and ___ cells through the release of TGF-beta
Th1 and TH2
effector functions of Th1 cells are
- IFN-gamma and CD40 ligand
- Fas ligand or LTbeta
- IL2
- IL3 + GM-CSF
- TNF-alpha +LTalpha
- CXCL2
What does Fas ligand or LTbeta do
kills chronically infected cells, releasing bacteria to be destroyed by other macrophages
What does IL-2 do?
- induce T cell proliferation
- NK cells CTL
What does IL3 +GM-CSF do?
induce macrophage differentiation in bone marrow
What does TN-alpha + LT alpha do?
activate endothelium
What does CXCL2 do?
attracts macrophages to site of infection
IL2 produced by ___ activate ___ and ____
CD4+ T cells
CD8+ T cells and NK cells
CD4+ cells can provide feedback signals to DCs though ____ and _____ leading to release of ___ or ____
IFN-gamma and CD40 L
IL10 or IL12
Effector cells can respond to target cells ______ stimulation
without
