Lecture 15 - Enzymes, Kinetics, Inhibition & Control

Question 1

an enzyme modifies _____

Answer 1

the reaction barrier

Question 2

what are 2 models of enzymes?

Answer 2

1. lock and key

2. induced fit

Question 3

what does a typical enzyme kinetics experiment measure?

Answer 3

-initial rate (v0) as a function of substrate concentration [S]

Question 4

What does the kinetics curve of an enzyme reaction look like?

Answer 4

a saturation binding curve

Question 5

why does an enzyme reaction curve look similar to a saturation binding curve?

Answer 5

-because “turnover” is preceded by substrate/enzyme binding

Question 6

What is turnover controlled by?

Answer 6

catalytic efficiency

Question 7

what is enzymatic activity revealed by?

Answer 7

the rate of the production of P (the velocity of the reaction)

Question 8

Enzyme kinetics is a function of ________ and _______ efficiency

Answer 8

• binding

- catalytic

Question 9

what is Km?

Answer 9

measure of enzyme-substrate binding affinity

Question 10

what is Vmax?

Answer 10

measure of catalytic efficiency

Question 11

[E]total =

Answer 11

[E] + [ES]

Question 12

V0 =

Answer 12

Vmax[S]/Km+[S]

Question 13

what is the interpretation of the Michaelis-Menten equation?

Answer 13

when [S]&raquo_space; [E]T, S saturates all the binding sites.

Vmax is proportional to [E]Txthe catalytic efficiency

Question 14

kcat =

Answer 14

Vmax/[E]total

Question 15

Linewaver-Burk plots

Answer 15

?