lecture 15

Question 1

what is function of micro arrays?

Answer 1

measure gene expression/hybridisation

Question 1

what is illuminated sequencing?

Answer 1

fragments of DNA bound to solid surface, modified nucleotides with fluroescent group block extension to allow only 1 base to be added per cycle

Question 2

what is measured in an illumina sequencing reaction?

Answer 2

the colour of light emitted from the reaction, as each base has its own coloured label

Question 3

what is RNA sequencing?

Answer 3

mRNA converted to cDNA, which is then used for sequencing library generation, allows quantification, profiling + discovery o RNA

Question 4

RNA sequencing vs microarray

Answer 4

RNA sequencing >, direct sequencing, easier, better results

Question 5

ChIP sequencing

Answer 5

cross link proteins to DNA, isolate DNA and shear

Question 6

what is ATAC-sequencing?

Answer 6

Assay for Transposase-Accessible Chromatin using sequencing

Question 7

what is Bisulphite sequencing?

Answer 7

Bisulphite treatment causes the unmethylated cytosines to be converted to uracil, which is read as thymine during sequencing

Question 8

what is reduced-representation sequencing?

Answer 8

uses Mspl restriction enzymes to enrich for CpGs only need to sequence 1% of genome