LECTURE 14 and 15

Question 1

how is genomic sequence obtained?

Answer 1

1. obtain organisms genomic DNA
2. break DNA into small fragments
3. obtain DNA sequence from fragments
4. search for overlaps identity

Question 2

why use model organisms?

Answer 2

small genome - value for money
easy to manipulate
provide info on fundamental biological processes
technology development

Question 3

what happens with genomic DNA?

Answer 3

transcribed with mENA
intron removed

Question 4

E.coli infections 2011

Answer 4

an unidentified strain of E.coli killed 50 europeans
genome was sequenced in 2 days

Question 5

the Black Death

Answer 5

Yersinia pestis strain killed 30 million europeans in 14th century
sequencing the genome from teeth of victims showed a new strain

Question 6

plague of Justinian

Answer 6

Yersinia pestis strain killing 100 million contributing to the end of the Roman Empire

Question 7

what are the Functional Characterisation of Mutant Proteins ?

Answer 7

Analysis of predicted catalytic mutant Msh2 proteins from human colon cancer was confirmed by expressing the proteins in yeast.

Question 8

what did analysis of other mutant Msh2 proteins reveal?

Answer 8

Defects in critical protein-protein interactions

Reduced steady state levels of Msh2

Mutations affected the activity of the mismatch repair complex

Question 9

PSORTII programme

Answer 9

allows predictions to be made as to localisation of the gene
experiments have to be carried out to confirm prediction

Question 10

medical applications of the use of computers in genome research

Answer 10

genetics and personalised medicine

Question 11

major issues with use of computers in genome research

Answer 11

ethics
genetic counselling