LECTURE 12 Control of SM Gene Expression by Epigenetics Flashcards
Activation Methylation Mark
H3K4
Repression Methylation Marks
- H3K9 me3 recruits Heterochromatin protein HP1
- H3K27 me3
- H4K20
What is the COMPASS complex
COMPASS complex is a multiprotein complex containing histone methyltransferases with SET domain
what does this complex contains
the SET1 methyltransferase
SET1 methyltransferase
di- and trimethylates lysine 4 in histone H3 (H3K4me2/3)
what is H3K4 associated with
gene activation at euchromatic regions
What does the SET domain consist of?
130 amino acids conserved catalytic domain where two motifs RFINHXCXPN and ELFXFDY are necessary for the function of the enzyme
what is H3K36 me3 mediated by
SET2 methyltransferase
Set1–COMPASS Functions
Sole enzyme responsible for monomethylation, dimethylation, and trimethylation of H3K4 in yeast.
Set1–COMPASS Identification
Set1–COMPASS was identified as the pioneering member of the H3K4 methyltransferases in yeast.
Set1–COMPASS Association
Set1–COMPASS is associated with the methylation of histone H3 at lysine 4 (H3K4) within chromatin, affecting gene expression and chromatin structure.
cclA and setA Deletions in Fungi Phenotypes
Deletion of cclA and setA subunits results in similar outcomes: impaired growth, brown pigmentation, and absence of sexual fruiting bodies.
cclA and setA Deletions in Fungi Effects
Deletion of cclA activates the production of secondary metabolites, such as monodictyphenone (antimicrobial) and emodin derivatives (demonstrating various beneficial activities).
Effect of clrD and hepA Deletions in A. nidulans: Morphology
Deletion of clrD and hepA genes does not cause observable morphological changes in A. nidulans.
Effect of clrD and hepA Deletions in A. nidulans: Deletion Impact
Removing clrD and hepA results in the derepression of Sterigmatocystin and Penicillin gene expression and production.
