Lecture 10

Question 1

True or false: Viruses infect all 3 domains of life

Answer 1

True

Question 2

True or false: A large amount of viruses are pathogenic

Answer 2

False
-Only a small amount of is pathogenic

Question 3

What is a plaque?

Answer 3

Clear zones that develop on lawns of host cells
-where bacteria has been killed

Question 4

How can we classify phages?

Answer 4

Morphology

Question 5

Caudovirales

Answer 5

tailed dsDNA
=95% of phages

Question 6

Siphoviralde

Answer 6

long tails that don’t contract
-61% of phages

Question 7

Myoviridae

Answer 7

contractile tails w/ a sheath and central tube
-25% of phages

Question 8

Podoviridae

Answer 8

short non contractile tails
-14% of phages

Question 9

How can we see viruses?

Answer 9

Plaque assay and electron microscopy

Question 10

What are the parts of bacteriophage structure

Answer 10

1. Head–>nucleic acid and capsid
2. Tail–> tail sheath
3. Tail fibers

Question 11

What type of nucleic acids do viruses consist of?

Answer 11

-dsDNA
-ssDNA
-ssRNA
-dsRNA

Question 12

What kind of nucleic acids do bacteriophages have?

Answer 12

dsDNA

Question 13

True or false: not all viruses have capsids

Answer 13

False
All viruses have capsids

Question 14

What is a capsid

Answer 14

A protein shell that protects nucleic acid

Question 15

What is the most common shape for a capsid

Answer 15

Icosahedral

Question 16

Why is icosahedral the best shape for a capsid?

Answer 16

requires the smallest number of capsomeres to build the shell

Question 17

What is 35S?

Answer 17

Binds to PROTEINS and not DNA

Question 18

What is 32P?

Answer 18

Binds to DNA and not proteins