Lecture 1 Old Notes: Tandler Flashcards
What is resolution and how does it relate to magnification?
It is about the ability to see fine detail and is independent of magnification
What are the two most common stains used for histologic presentation?
Hematoxylin and Eosin
Why does the nucleus tend to stain with basic dyes?
It is basophillic due to the nucleus being an acidic environment
Why does the cytoplasm of a cell tend to react with acidic dyes?
It has pKs around 8, making it slightly basic.
Is hemotoxylin basic or acidic?
Basic, so used to stain nuclei
Is eosin acidic or basic?
Acidic, so it stains cytoplasm
What cellular structure contains all of the enzymes involved in oxidative phosphorylation?
Mitochondria
How many different enzymes does mitochondria contain?
Over 120
For the mitochondrial membranes, which one is folded and which is flat?
Inner is folded, outer is flat
What shape are mitochondrial cristae in the adrenal glands, testes or ovaries?
Tubular
How common are mitochondrial disturbances?
1:2000
What material does smooth ER help digest and synthesize?
Glycogen
What kind of proteins comprise the particles on the outside of rough ER?
Ribonucleo proteins
Are ribonucleo proteins acidic or basic?
Acidic
What takes amino acids and incorporates them into proteins?
Cisternae