Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Microbiology test 2 > Lect 13 > Flashcards

Lect 13 Flashcards

1
Q

Haemophilus influenzae

  • gram status
  • motility
  • shape
A
  • gram negative
  • non-motile
  • coccobacillus
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Haemophilus influenzae has two different strains: describe them

A
  1. encapsulated strains
  2. non-encapsulated strains: non-typeable
    • part of normal flora
    • still capable of causing disease
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Haemophilus influenzae virulence factors

A
  • PRP capsule
  • neuraminidase and a IgA protease
  • Fimbriae
  • LOS
    • lipooligosaccharide
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

type B Haemophilus influenzae is responsible for what disease

A
  • PNA in infants and young children
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

non-encapsulated Haemophilus influenzae strains possess what that bind to mucins on the ciliated epithelial cells of the respiratory tract

A

adhesins (HAP)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

In Haemophilus influenzae infection, loss of cilia, inflammation, and sloughing of damaged epithelial cells all result from secretion of what virulence factor

A

LOS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

describe Haemophilus influenzae growth on a blood agar

A
  • blood agar growth is only acheived as a satellite phenomenon around other bacteria
  • colonies of Haemophilus influenzae appear as convex, smooth, pale, grey or transparent colonies
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Haemophilus influenzae has what result on catalase and oxidase tests

A

positive

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Haemophilus influenzae detection is done via what test

A

ID-latex particle agglutination test (LAT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

treatment of Haemophilus influenzae

A
  • B-lactams (amoxicillin/clavulanic acid)
  • if resistance, or highly invasive infection, use 3rd generation cephalosporin
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

90% of all human infections resulting from legionella pneumophilia are from what serogroup

A

serogroup 1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

legionella pneumophilia

  • gram status
  • shape
A
  • gram negative
  • pleomorphic bacillus
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

how do legionella pneumophilia survive

A
  • survive intracellularly
    • inhibit phagolysosome fusion
    • phagosome surroundes itself with ER studed with ribosomes
    • replicate inside phagosome
    • host cell is killed when the phagosome lyses, releasing toxic enzymes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

legionella pneumophilia infection results in one of what two diseases

A
  • pontiac fever
  • Legionnaire’s disease
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

describe pontaic fever

A
  • caused by legionella pneumophilia
  • self limited illness
    • ​resolve spontaneously without treatment
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

describe Legionnaires’ disease

A
  • caused by legionella pneumophilia
  • a severe, acute atypical PNA with a high mortality rate (75% w/o treatment)
  • CAP
17
Q

Describe how Legionnaires diseases goes from infection -> PNA

A
  1. entry of bacilli into macrophages
  2. multiplication of bacteria in macrophages
  3. death of macrophages
  4. release of chemotactic factors
  5. influx of monocytes and PMNs
  6. acute fibropurulent necrotizing PNA
18
Q

legionella pneumophilia is found in what type of environements

A

moist environments

19
Q

in nature, legionella pneumophilia survive and replicate within what

A

protozoans

20
Q

transmission of legionella pneumophilia

A
  • aerosols
  • no person to person transmission
21
Q

risk factors for legionella pneumophilia

A
  • any compromise in pulmonary and/or immune function
    • smoking
    • chronic lung or heart dz
    • immunosuppression
    • elderly
    • alcoholics
22
Q

how is legionella pneumophilia diagnosed

A
  • culture on Buffered charcoal yeast extract (BCYE) - gold standard
  • rapid test for antigen in urine
    • EIA test
23
Q

DOC treatment for legionella pneumophilia-> legionnaires

A
  • DOC: levofloxacin
24
Q

psuedomonas aeruginosa

  • gram status
  • shape
  • oxygen required?
  • motile?
A
  • gram negative
  • bacillus
  • aerobic
  • motile: single flagellum
25
Q

psuedomonas aeruginosa is blue-green in color due to the production of what two pigments

A
  • Pyoverdin: green
  • Pyocyanin:blue; virulence factor
26
Q

function of pyocyanin psuedomonas aeruginosa

A
  • blue pigment
  • catalyzes ROS production - tissue damage
27
Q

list the virulence factors of psuedomonas aeruginosa

A
  • pyocyanin
  • Endotoxin A-B
  • Elastases
  • Alginate
  • LPS
28
Q

function of alginate in psuedomonas aeruginosa

A
  • mucoid polysaccharide/slime layer
    • adherence
    • inhibits mucociliary escalator
      • important in CF (cystic fibrosis)
    • inhibits complement and antibody binding
29
Q

transmission of psuedomonas aeruginosa occurs via

A
  • fomites
  • plants
  • fruits
  • hands
30
Q

what media support growth of psuedomonas aeruginosa? Expected results?

A
  • BAP
  • MacConkey
  • produce blue-green pigment
  • may tinge sputum or pus
31
Q

treatment of psuedomonas aeruginosa NP infections

A
  • combo
    • Cefepime (4th gen ceph) + Levofloxacin (fluoroquinolone)

Microbiology test 2 (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions