LEC review: blood collection equipment Flashcards
The primary duty of the phlebotomist is not to collect blood specimens for laboratory testing
true or false
false
incomplete equipment ensures orderly collection of blood samples for testing
true or false
false
area of a medical laboratory or clinic equipped for performing phlebotomy procedures on patients
Blood-Drawing Station
Phlebotomy Chairs should be comfortable for the patient and have adjustable armrests to achieve
proper positioning
equipment carrier used for “stat” or emergency situations
handheld carrirer
equipment carrier used for early-morning extractions which is potential for nosocomial infections
phlebotomy cart
prevent or inhibit the growth and development of microorganisms but do not necessarily kill them
antiseptic
most common antiseptic
70% isopropanol
antiseptic used for a higher degree
of antisepsis
povidone-iodine
which Antispetic is not used in Blood Collection: 70% Ethanol 70% Isopropanol Benzalkonium chloride saline solution Chlorhexidine gluconate Hydrogen peroxide Povidone-iodine Tincture of iodine
saline solution
are chemical substances or solutions that are used to remove or kill microorganisms on surfaces and instruments
disinfectant
most common disinfectant
sodium hypochlorite
fresh bleach solution should be made daily or as needed
true or false
true
are used to hold pressure over the site following blood collection procedure
Gauze pads / Cotton balls
are used to cover a blood collection site after the bleeding has stopped
bandages
Must be rigid, puncture-resistant, leakproof, and disposable and have locking lids
Sharps disposal containers
Sharps containers can be overfilled
true or false
false
are leakproof plastic bags that are commonly used to transport blood and other specimens from the collection site to the laboratory
Biohazard Bags
are used to make blood films for hematology determinations
glass microscope slides
make it easier to locate veins that are difficult to see or feel
Transillumination devices
applied or tied around a patient’s arm prior to venipuncture to restrict blood flow
Tourniquet
Restriction of venous flow distends or inflates the veins,
making them larger and easier to find, and stretches the vein
walls so they are thinner and easier to pierce with a needle
tourniquet applied can be longer than 1 min
first is true, second is false
blood pressure cuff should not be used in place of a tourniquet
true or false
false
are sterile, disposable, and designed for a single use only
needles
3 types of needles:
- Hypodermic
- Multisample
- Winged infusion (butterfly)
is indicated by a number that is related to the diameter of the lumen
Needle Gauge
considered the standard for most routine adult antecubital venipuncture
21-gauge needle
A needle’s diameter and gauge have an ________ relationship
inverse
must provide immediate permanent containment and be activated using one hand
needle safety feature
3 examples of needle safety feature:
sliding sheath
hinged arm / snap down
retracting needle
closed system
ETS
basic system of ETS:
needle, tube holder, evacuated tubes
is a clear, plastic, disposable cylinder with 2 openings
- Small threaded opening: for needle
- Large opening: for tubes
tube holder
are used with both the ETS and the syringe method volume range: 1.8 to 15 mL
evacuated tubes
negative pressure of evacuated tubes
vacuum
A tube that has prematurely lost all or part of its vacuum will properly fill with blood
true or false
false
premature loss of vacuum except:
- Improper storage
- Dropping the tube
- improper storage angle of tube
- Advancing the tube too far onto the needle before venipuncture
- The bevel becomes partially out of the skin during venipuncture
- improper storage angle of tube
reasons for short draw except:
- Premature loss of vacuum
- improper tube selection
- Removing the tube prior vacuum exhaustion
- Stoppage of blood flow during blood draw
- improper tube selection
any substance placed within a tube other than the tube stopper
tube additive
clot activator yields
serum
prevent clotting yields
whole blood specimen
tubes used for clearing or discard purposes
non additve tubes - yields serum samples
used for patients with small or difficult veins
syringe system
rod-like device that fits tightly into the barrel
plunger
For small or difficult veins (hand veins) Allows more flexibility and precision
butterfly/winged infusion set
most common anticoagulant prevents
clotting
2 methods of anticoagulation
(1) chelating/binding
(2) precipitating calcium
- commonly available as a powdered di-potassium (K2) or liquid tri-potassium (K3) salt
- prevents coagulation by
binding or chelating
calcium - for hematology; preserve cell morphology
- inhibit platelet aggregation
- invert 8 - 10 times
EDTA - ethylenediaminetetraacetic acid
- Used for coagulation tests
- 9:1 ratio of blood to anticoagulant
- invert 3-4
- binding or chelating calcium
citrate
- prevents clotting by inhibiting thrombin formation
- For some chemistry tests
heparin
3 forms of heparin:
ammonium
lithium (least interference)
sodium
- prevent coagulation by precipitating calcium
- contain glucose preservatives
- Inversions: 8 to 10
oxalate
- For immunohematology tests
- Binds calcium
- Yellow-topped
- Inversions: 8
Acid Citrate Dextrose
- For blood transfusion
- Chelates calcium
Citrate Phosphate Dextrose
- Binds calcium
- For blood culture
- Yellow-topped
- Inversions: 8
Sodium Polyanethol Sulfonate
- Antiglycotic agent
- Used for ethanol specimens
- Gray-topped
- Inversions: 5 to 10
Sodium Fluoride
is a substance that enhances coagulation in tubes used to collect serum specimens
Clot Activator - silica (glass) or celite (inert clay)
- an inert (nonreacting) synthetic substance initially contained in or near the bottom of certain blood collection tubes
- prevents the cells from continuing to metabolize substances such as glucose
Thixotropic Gel
ORDER OF DRAW
stop light red stay put green light go
sterile, light blue, red, SST, PST, green, lavender, gray
boys like ravishing girls like dieticians love greek yougurt
blood culture, light blue, gold, light green, dark green, lavender, grey, yellow
Filling tubes in the wrong order can lead to interference in testing from cross contamination of the specimen by additive carryover, tissue thromboplastin, or microorganisms
true or false
true
- transfer of additive from one tube to the next
- occurs by: when blood in an additive tube touches the needle
during ETS blood collection or when blood is transferred
from a syringe into ETS tubes
Carryover/Cross-Contamination