Lec 35 Gas Diffusion Flashcards
What is general gas law?
PV = nRT in BTPS P = pressure in mmHg V = volume in L n = number of moles R = gas constant T = temperature
What is BTPS?
Body Temp: 37 C or 310 K
Ambient P
Saturated with water vapor
What is water vapor pressure at T = 37 C?
47 mmHg
When do you use BTPS?
gas phase
When do you use STPD?
liquid phase [gas dissolved in blood]
What is STPD?
Standard Temp = 0 C, 273 K
Standard P = 760 mmHg
Dry gas
What is standard temp
0 C, 273 K
What is standard pressure
760 mmHg
What is boyle’s law?
- special case of general gas law
- at constant temp: P1V1 = P2V2
Example of boyle’s law in inspiration?
inspiration –> diaphragm contracts –> get increase lung volume –> get decreased gas pressure to keep PV constant –> decreased P is driving for for airflow into lung
What is partial pressure for dry gas x?
Px = Pb * F Pb = barometric pressure F = fractional concentration
What is partial pressure for humidified gas x?
Px = (Pb - Ph2o) * F Pb = barometric pressure F = fractional concentration
What is definition of partial pressure of gas x?
the pressure gas x would exert if it occupied total volume of mixture
What is daltons law?
total pressure of mixture of gases = sum of partial pressures of all gases in the mixture
What is barometric pressure equation?
Pb = Po2 + Pco2 + Pn2 + Ph2o
What is percent of O2/N2/CO2 gases in dry air at barometric pressure 760 mmHg?
O2: 21%
N2: 79%
CO2: 0%
What is Ph2o in humidified tracheal air at 37 C?
47 mmHg
What happens to dalton’s law at high altitude?
have reduced barometric pressure –> decreased PO2 of inspired air –> lower alveolar PO2
What is henry’s law? when does it apply?
- applies for concentration of dissolved gas free in solution [not bound to plasma proteins or Hb]
- Cx = Px * S
Cx = concentration of dissolved gas ml/100 mL blood
Px = partial P gas
S = solubility gas in blood mL/100 mL/mmHg
At equilibrium what is relationship partial P gas in liquid and gas phases?
partial P gas in liquid = partial P gas in gas phase
At equilibrium what is alveolar air PO2? pulm capilllary blood PO2?
both are 100 mmHg
What dissolved gas does not apply in henry’s law concentration? what dissolved gas is included?
dissolved gas that is bound to plasma proteins or Hb not included
only dissolved gas that is free is included
How do you calculate concentration of dissolved O2?
concentration dissolved O2 = PO2 * solubility
What is solubility of O2?
0.003 mL O2/ 100 mL blood/ mmHg
What is the concentration of dissolved O2 in arterial blood?
0.3 mL/ 100 mL
How are gases transferred across cell membranes / capillaries?
simple diffusion
What 4 factors affect rate of diffusion of gases?
- driving force [partial pressure difference]
- diffusion coefficient
- surface area available
- thickness of barrier
What is Fick’s law?
Vx = DAΔP / ΔX Vx = rate of transfer of gas by diffusion [Vx = vol of gas trasnferred per unit time] D = diffusion coefficient A = surface area ΔP = partial pressure difference [ driving force] ΔX = thickness of membrane barrier
In fick’s law what 3 things are directly proportional to Vx? What 1 thing is inversely proportional to Vx?
directly - ΔP: driving force, partial pressure difference of gases - D: diffusion coefficient - A: surface area indirectly - ΔX: thickness of membrane barrier
What is the driving force for diffusion of a gas? What is the value in normal alveoli diffusion
partial pressure difference of gas across the membrane ΔP
PO2 alveolar - PO2 mixed venous blood = 100 - 40 = 60 mmHg
What 2 things does diffusion coefficient D of a gas depend on?
- molecular weight
- solubility
Does CO2 or O2 have higher D?
CO2 had 20 x higher D than O2
Does CO2 or O2 diffuse faster?
CO2 diffuses 20x faster than O2 at any given partial pressure
What is total gas content in soln [blood] sum of [3 things]?
dissolved gas + gas bound to protein + chemically modified gas
Is the amount of gas dissolved in solution directly or indirectly proportional to pressure of gas in solution?
directly proportional
At given partial pressure, higher solubility does what to concentration?
higher solubility –> high concentration
Which gas is exclusively carried in dissolved form?
N2
What forms of bound O2 in solution?
- O2 bound to Hb inside RBCs
What forms of bound CO2 in solution?
- CO2 bound to both Hb inside RBCs and to plasma proteins
What percent of CO2 chemically modified? how is it modified?
- 90% of CO2 converted to H2CO3 by carbonic anhydrase in RBC
- H2CO3 –> H+ + HCO3-
What percent of CO2 dissolved in plasma as CO2?
7%
What percent of CO2 bound to Hb in RBC?
3%
What is PO2 and PCO2 in dry inspired air?
PO2 = 160 PCO2 = 0
What is PO2 and PCO2 in humidified tracheal air?
PO2 = 150 PCO2 = 0
What is PO2 and PCO2 in alveoli?
PO2 = 100 PCO2 = 40
What is PO2 and PCO2 in mixed venous blood?
PO2 = 40 PCO2 = 46
What is PO2 and PCO2 in systemic arterial blood?
PO2 = 100 PCO2 = 40
What is cause of discrepancy between alveolar air PO2 and systemic arterial blood PO2?
2% of cardiac output bypasses alveoli via physiologic shunts –> bronchial blood flow and coronary venous return
What is PO2 and PCO2 in peripheral tissues?
PO2 = 40 mmHg PCO2 = 46 mmHg
What is diffusion-limited gas exchange across alveolar-capillary barrier?
- total amount gas transported across alveolar-capillary barrier is limited by diffusion process
- as long as partial P gradient maintained, diffusion will continue along length of capillary
What 3 things can bind Hb inside RBCs
- O2
- CO
- CO2
What are 4 examples of diffusion limited gas exchange?
- transport of CO: because CO binds Hb as it enters blood so doesn’t create partial pressure in blood so never equilibrates
- transport O2 in emphysema: destruction of alveoli wall so smaller surface area so decreased diffusion
- transport O2 in pulm fibrosis: increased thickness so much slower diffusion
- transport O2 in exercise: increased blood flow so diffusion becomes limit
What is limited in diffusion of CO?
diffusion limited
What is limited in diffusion of O2 in emphysema?
diffusion limited
What is limited in diffusion of O2 in pulmonary fibrosis?
diffusion limited
What is limited in diffusion of O2 in exercise?
diffusion limited
What is perfusion limited gas exchange?
- total amount of gas transported across alveolar/capillary barrier is limited by blood flow [perfusion]
- partial pressure gradient is not maintained, only way to increase amount of gas transported is by increasing blood flow
In what kind of limited gas exchange is partial pressure maintained? not maintained?
- maintained in diffusion limited gas exchange
- not maintained in perfusion limited gas exchange
What are 3 examples of perfusion limited gas exchange?
- O2 under normal conditions
- CO2
- N2O [since highly dissolved and not bound]
Where does pressure gradient become equal in perfusion limited gas exchange in capillary?
in the first 1/3 of capillary
What is limited in diffusion of O2 in normal conditions?
- perfusion limited
- gradient for diffusion maintained in beginning b/c O2 binds Hb first so not
- as dissolved O2 increases, so does blood PO2
What is limited in diffusion of CO2 in normal conditions?
perfusion limited
What is limited in diffusion of N2O in normal conditions?
perfusion limited
What is significance of O2 doing all its diffusion in first 1/3 of capillary?
its now perfusion limited so theres lots of room to increase diffusion by increasing blood flow in exercise
What is used to measure DL [diffusion capacity of lung]? Why?
- CO is used
- because its transfer is exclusively limited by diffusion process since it binds Hb as soon as it enters blood it basically has no dissolved partial pressure