lec 3. neural induction

Question 1

non-neural homeobox genes (in vertebrates and invertebrates)

Answer 1

BMP1, BMP4, BMP7

Tolloid, Decapentalegic, Screw

Question 2

neural genes (in vertebrates and invertebrates)

Answer 2

Chordin
Short Gastrulation (Sog)

Question 3

Chordin

Answer 3

released by organiser cells (node) in mesoderm and diffuse into ectoderm to STOP BMP signalling to allow neural induction

Question 4

neural induction

Answer 4

ectoderm acquires neural fate after BMP signal is inhibited by Chordin

Question 5

spemann and mangold organiser

Answer 5

transplanted donor organiser into opposite side of host embryo which resulted in two axes (proof of neural induction by organiser)

Question 6

how BMP antagonist were discovered

Answer 6

ALL mRNA was extracted from organiser cells, reverse transcribing into cDNA, then testing each to look for a protein that would mimic organiser ability to induce a secondary neural plate

Question 7

in situ hybridisation

Answer 7

make antisense probe (attached to fluorescent indicator) to gene of interest to find it in a tissue