LEC 2

Question 1

AST is formerly known as:

Answer 1

Serums Glutamic Oxaloacetic transaminase

Question 2

What are the 2 products of the 2 products of the forward reaction catalyzed by AST?

Answer 2

Oxaloacetate
Glutamate

Question 3

What is the systematic name of AST?

Answer 3

E. C. 2.6.1.1

Question 4

What are the two isoenzymes of AST?

Answer 4

Cytoplasmic
Mitochondrial

Question 5

In severe tissue damage, what isoenzyme will predominately increase?

Answer 5

Mitochondrial

Question 6

In AMI. AST levels will return to normal win how many days?

Answer 6

5 days

Question 7

In cases of cirrhosis, AST level may increase about how many times the ULN value?

Answer 7

4x

Question 8

Following viral hepatitis, AST level may increase about how many times the ULN value?

Answer 8

100x

Question 9

AST level is not useful in the diagnosis of AMI. True or False

Answer 9

True

Question 10

What is the systematic name of ALT?

Answer 10

E. C. 2.8.1.2

Question 11

What are the two substrate of the reaction catalyzed by ALT?

Answer 11

Alanine
Alpha-ketoglutarate

Question 12

ALT is formerly known as:

Answer 12

Serum Glutamic Pyruvic transaminase

Question 13

This is also known as ALT/AST ration

Answer 13

De Ritis Ratio

Question 14

The Karmen method monitors absorbance at what wavelength?

Answer 14

340 nm

Question 15

What is the indicator enzyme for the AST assay following Karmen method?

Answer 15

Malate Dehydrogenase

Question 16

What are the two products of your answer in No. 18?

Answer 16

Malate
NAD

Question 17

Hemolvsis when measuring AST must be avoided since; AST in RBCs is (?)
times greater than the serum.

Answer 17

15x

Question 18

What is the indicator enzvme in ALT?

Answer 18

LDH

Question 19

What are the two product of the reaction catalyzing by your answer in No. 22?

Answer 19

Lactate
NAD

Question 20

ALT is a more liver specific enzyme than AST. True or False

Answer 20

True

Question 21

What is the systematic name of CK?

Answer 21

E. C. 2.7.3.2

Question 22

What are the products of the reverse reaction catalyzed by CK?

Answer 22

Creatine
ATP

Question 23

What is the optimum pH of the forward reaction catalyzed by Ck?

Answer 23

9.0

Question 24

This is considered the major phosphorylated compound in muscle

Answer 24

Phosphocreatine

Question 25

This is an obligate activator the reaction catalyzed by CK

Answer 25

Magnesium

Question 26

What CK isoenzyme predominates the skeletal and cardiac muscle?

Answer 26

CK MM

Question 27

What CK isoenzyme is also used as a tumor marker?

Answer 27

CK BB

Question 28

What CK isoenzyme constitute up to 15% of Ck activity in the heart?

Answer 28

CK Mt

Question 29

What CK isenzyme is elevated in AMI?

Answer 29

CK MB

Question 30

What CK isoenzyme is elevated in hypothyroidism

Answer 30

CK MM

Question 31

Enumerate the three enzymes involved in the Oliver Rosalki method.

Answer 31

CK
HK
G6PD

Question 32

This is the reference method for the quantitation of CK isoenzymes.

Answer 32

Electrophoresis

Question 33

What is the systematic name of LDH?

Answer 33

E. C. 1.1.1.27

Question 34

What is the hydrogen acceptor of LDH activity?

Answer 34

NAD

Question 35

This isoenzyme of LDH is also known as alcohol dehydrogenase

Answer 35

LDH6

Question 36

What isoenzyme of LDH is increased in Carcinoma?

Answer 36

LDH3

Question 37

What is the pH of the Wacker method?

Answer 37

8.8

Question 38

Isoenzyme of LDH mainly found in the liver.

Answer 38

LDH4

Question 39

Isoenzyme of LDH elevated in AMI.

Answer 39

LDH1

Question 40

Isoenzyme of LDG elevated in skelelai muscite injury.

Answer 40

LDH5

Question 41

What is the optimal pH of the forward reaction of LDH?

Answer 41

8.8 to 9.8

Question 42

Most abundant LDH isoenzyme in normal blood.

Answer 42

LDH2