LEC 2 Flashcards
AST is formerly known as:
Serums Glutamic Oxaloacetic transaminase
What are the 2 products of the 2 products of the forward reaction catalyzed by AST?
Oxaloacetate
Glutamate
What is the systematic name of AST?
E. C. 2.6.1.1
What are the two isoenzymes of AST?
Cytoplasmic
Mitochondrial
In severe tissue damage, what isoenzyme will predominately increase?
Mitochondrial
In AMI. AST levels will return to normal win how many days?
5 days
In cases of cirrhosis, AST level may increase about how many times the ULN value?
4x
Following viral hepatitis, AST level may increase about how many times the ULN value?
100x
AST level is not useful in the diagnosis of AMI. True or False
True
What is the systematic name of ALT?
E. C. 2.8.1.2
What are the two substrate of the reaction catalyzed by ALT?
Alanine
Alpha-ketoglutarate
ALT is formerly known as:
Serum Glutamic Pyruvic transaminase
This is also known as ALT/AST ration
De Ritis Ratio
The Karmen method monitors absorbance at what wavelength?
340 nm
What is the indicator enzyme for the AST assay following Karmen method?
Malate Dehydrogenase
What are the two products of your answer in No. 18?
Malate
NAD
Hemolvsis when measuring AST must be avoided since; AST in RBCs is (?)
times greater than the serum.
15x
What is the indicator enzvme in ALT?
LDH
What are the two product of the reaction catalyzing by your answer in No. 22?
Lactate
NAD
ALT is a more liver specific enzyme than AST. True or False
True
What is the systematic name of CK?
E. C. 2.7.3.2
What are the products of the reverse reaction catalyzed by CK?
Creatine
ATP
What is the optimum pH of the forward reaction catalyzed by Ck?
9.0
This is considered the major phosphorylated compound in muscle
Phosphocreatine
This is an obligate activator the reaction catalyzed by CK
Magnesium
What CK isoenzyme predominates the skeletal and cardiac muscle?
CK MM
What CK isoenzyme is also used as a tumor marker?
CK BB
What CK isoenzyme constitute up to 15% of Ck activity in the heart?
CK Mt
What CK isenzyme is elevated in AMI?
CK MB
What CK isoenzyme is elevated in hypothyroidism
CK MM
Enumerate the three enzymes involved in the Oliver Rosalki method.
CK
HK
G6PD
This is the reference method for the quantitation of CK isoenzymes.
Electrophoresis
What is the systematic name of LDH?
E. C. 1.1.1.27
What is the hydrogen acceptor of LDH activity?
NAD
This isoenzyme of LDH is also known as alcohol dehydrogenase
LDH6
What isoenzyme of LDH is increased in Carcinoma?
LDH3
What is the pH of the Wacker method?
8.8
Isoenzyme of LDH mainly found in the liver.
LDH4
Isoenzyme of LDH elevated in AMI.
LDH1
Isoenzyme of LDG elevated in skelelai muscite injury.
LDH5
What is the optimal pH of the forward reaction of LDH?
8.8 to 9.8
Most abundant LDH isoenzyme in normal blood.
LDH2
