LAB 2

Question 1

What is the systematic name of AST?

Answer 1

E. C. 2.6.1.1

Question 2

What are the products of the reverse reaction catalyzed by AST?

Answer 2

Aspartate
Alpha-ketoglutarate

Question 3

In the procedure performed for AST, what was the volume of the sample?

Answer 3

125 uL

Question 4

What is the systematic name of ALT?

Answer 4

E. C. 2.6.1.2

Question 5

What are the products of the forward reaction catalyzed by ALT?

Answer 5

Pyruvate
Glutamate

Question 6

In the procedure performed for ALT, what was the volume of reagent 1?

Answer 6

1000 uL

Question 7

In the procedure performed for ALT, how long was the incubation after the addition of reagent 2?

Answer 7

90s

Question 8

Complete the Principle of the test for LDH: Lactate dehydrogenase with a systematic name (21) catalyzes the conversion of Pyruvate to (22) in the presence of (29), which Is converted to (24). The rate of conversion is monitored at (25) wavelength and is proportional to LDH activity.

Answer 8

E. C. 1. 1. 1.27
Lactate
NADH
NAD
340 nm

Question 9

In the procedure performed for LDH, what was the volume of the sample?

Answer 9

1000 uL

Question 10

In the procedure performed for LDH, what was the volume of reagent 2?

Answer 10

10 uL

Question 11

LDH activity is stable for (?) days if samples are stored at 2-8C.

Answer 11

3-4 days

Question 12

What LDH Isoenzyme is the most labile?

Answer 12

LDH5

Question 13

What LDH isoenzyme is elevated in AMI?

Answer 13

LDH1