Lec 17

Question 1

What are the sections of a gene?

Answer 1

Promoter, coding region, termination signal

Question 2

Which sections of a gene code for the protein?

Answer 2

Only the coding region codes for the protein

Question 3

Going from the coding region to the termination signal, which direction is that?

Answer 3

Downstream

Question 4

The promoter is _______ from the coding region. (what direction?)

Answer 4

Upstream

Question 5

Is the promoter sequence transcribed?

Answer 5

No

Question 6

Is the termination signal transcribed?

Answer 6

Yes

Question 7

Where does transcription start?

Answer 7

+1

Question 8

Transcription in prokaryotes

Answer 8

Sigma with RNA polymerase has to recognize and bind -35 and -10 on the promoter

Question 9

Transcription in eukaryotes

Answer 9

Some transcription factors bind to DNA to guide RNA polymerase to promoter, others make DNA available to bind by removing chemical groups

Question 10

How does transcription end?

Answer 10

Termination signal is transcribed and folds back on itself in a hairpin, which causes mRNA and RNA polymerase to separate

Question 11

mRNA processing (only in eukaryotes)

Answer 11

Exons: regions of pre mRNA that code for a protein

Introns: regions of pre mRNA that don’t code for a protein

Splicing occurs, removes regions that don’t code for proteins (introns)