Lec. 16: Glycolysis / Gluconeogensis

Question 1

Generates the first molecule of ATP:

A) phosphoglycerate kinase

B) pyruvate kinase

C) phosphoglycerate mutase

D) phosphofructokinase

Answer 1

A) phosphoglycerate kinase

Question 2

Generates the second molecule of ATP:

A) phosphoglycerate kinase

B) pyruvate kinase

C) phosphoglycerate mutase

D) phosphofructokinase

Answer 2

B) pyruvate kinase

Question 3

Forms fructose 1,6-bisphosphate:

A) phosphoglycerate kinase

B) pyruvate kinase

C) phosphoglycerate mutase

D) phosphofructokinase

Answer 3

D) phosphofructokinase

Question 4

Converts 3-phosphoglycerate into 2-phosphoglycerate:

A) phosphoglycerate kinase

B) pyruvate kinase

C) phosphoglycerate mutase

D) phosphofructokinase

Answer 4

C) phosphoglycerate mutase

Question 5

Generates the first intermediate compound with a high phosphoryl-transfer potential that is not ATP:
(enzyme that catalyzes the redox (oxidation rxn))

A) enolase

B) glyceraldehyde 3-phosphate dehydrogenase

C) Phosphoglucose isomerase

D) aldolase

E) hexokinase

F) triose phosphate isomerase

Answer 5

B) glyceraldehyde 3-phosphate dehydrogenase

Question 6

Generates the second intermediate compound with a high phosphoryl-transfer potential:

A) enolase

B) glyceraldehyde 3-phosphate dehydrogenase

C) Phosphoglucose isomerase

D) aldolase

E) hexokinase

F) triose phosphate isomerase

Answer 6

A) enolase

Question 7

Converts glucose 6-phosphate into fructose 6-phosphate:

A) Phosphoglucose isomerase

B) aldolase

C) hexokinase

D) triose phosphate isomerase

Answer 7

A) Phosphoglucose isomerase

Question 8

Catalyzes the interconversion of three-carbon isomers:
(“kinetically perfect” enzyme)

A) Phosphoglucose isomerase

B) aldolase

C) hexokinase

D) triose phosphate isomerase

Answer 8

D) triose phosphate isomerase

Question 9

Cleaves fructose 1,6-biphosphate:

A) enolase

B) aldolase

C) hexokinase

Answer 9

B) aldolase

Question 10

Phosphorylates glucose:

A) enolase

B) aldolase

C) hexokinase

Answer 10

C) hexokinase

Question 11

Which of the following statements are true for muscle performing lactic acid fermentation?

The Process is exergonic

There is a net snthesis of NADH

The process is inhibited by ATP

The process is inhibited by AMP

Answer 11

The Process is exergonic

The process is inhibited by ATP

Question 12

Select all of the reactions of glycolysis in which ATP is produced.

A) phosphoenolpyruvate –> pyruvate

B) fructose 6 phosphate –> fructose 1,6-bisphosphate

C) Glucose –> glucose 6-phosphate

D) 1,3-Biphosphoglycerate –> 3-phosphoglycerate

E) Glyceraldehyde 3-phosphate –> 1,3-bisphosphoglycerate

Answer 12

A) phosphoenolpyruvate –> pyruvate

D) 1,3-Biphosphoglycerate –> 3-phosphoglycerate

Question 13

The first stage of glycolysis:

a. produces two molecules of ATP.

b. ends with the production of two molecules of dihydroxyacetone phosphate.

c. involves two phosphorylation reactions
and two isomerizations.

d. traps fructose in the cell.

e. forms a compound that can be readily cleaved into phosphorylated two- carbon units

Answer 13

c. involves two phosphorylation reactions
and two isomerizations.

Question 14

What enzyme catalyzes the rate determining step of glycolysis?

A. Hexokinase

B. Phosphoglucose Isomerase

C. Phosphofructokinase

D. Aldolase

E. Triose Phosphate Isomerase

Answer 14

C. Phosphofructokinase

Question 15

Which of the following best describes the
net organic products formed during the
oxidation of one molecule of glucose to
two molecules of pyruvate via glycolysis?

A. 1 ATP + 1 NADH
B. 2 ATP + 0 NADH
C. 2 ADP + 2 NAD+
D. 2 ATP + 2 NADH
E. 4 ATP + 2 NADH

Answer 15

D. 2 ATP + 2 NADH

Question 16

Why can’t cells continuously use the breakdown of glucose to pyruvate to generate energy without further conversion of pyruvate via fermentation or respiration?

A. The rate of energy production by glycolysis alone is too slow for cells’ biochemical reactions.

B. The amount of energy produced by glycolysis alone is insufficient to fuel cell growth.

C. The amount of inorganic phosphate available becomes limiting.

D. The electron acceptor reduced during glycolysis must be regenerated.

E. The complete breakdown of pyruvate is necessary to produce the carbon dioxide needed by cells

Answer 16

D. The electron acceptor reduced during glycolysis must be regenerated.

Question 17

Which is a means by which additional
sugars enter glycolysis?

a. Galactose is converted to fructose 6-phosphate.

b. Fructose in the adipose tissue is converted to glucose 6-phosphate.

c. Fructose in the liver is converted to dihydroxyacetone phosphate or glyceraldehyde 3- phosphate.

d. Galactose is converted to fructose 1,6 bisphosphate.

e. Lactose is converted to two molecules of fructose 6- phosphate

Answer 17

c. Fructose in the liver is converted to dihydroxyacetone phosphate or glyceraldehyde 3- phosphate.