Learning Outcomes - Week 8 - Muscle Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

Know the major functions of muscle and appreciate that there are three different types of muscle

A

A. The movement of the body (or parts thereof) through the environment. Examples of this function include the virtuosity of Brisbane classical guitarist Karen Schaupp or the power of surfer Molly Picklum .

B. The movements of materials through (or out of) the body. Movements of this type include the movement of blood through the cardiovascular system, the movement of digested food through the gastrointestinal tract, or the slightly less elegant expulsion of gastric contents during vomiting.

C. The maintenance of body temperature through heat production.

There are in fact three different types of muscle tissue in the human body.

The largest proportion (constituting around 40% of total body weight) is the muscle which, for the most part, attaches to the skeleton and is known as skeletal muscle.

The remaining muscle mass (making up 10% of body weight) consists of the muscle found in the heart (cardiac muscle) and smooth muscle which is found primarily in the walls of hollow structures such as blood vessels, the digestive tract and organs of the reproductive systems.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Describe the macro- and microscopic structure of skeletal muscle

A

Examination of a cross section through most skeletal muscles will reveal that it is spilt up into a number of compartments (known as fascicles) by connective tissue sheaths. Each of these fascicles is in turn made up of a large number of muscle cells which are the basic cellular units of a skeletal muscle. A single muscle cell is known as a muscle fibre. Each muscle fibre is formed during development by the fusion of a number of undifferentiated, mononucleated cells (known as myoblasts) into a single cylindrical, multinucleated cell. Adult skeletal muscle fibres are 10 to 100 micrometres in diameter, and can extend up to 20 cm in length.

As in other cells, the cell or plasma membrane of the muscle fibre (known as the sarcolemma) encloses the cytoplasm (known as sarcoplasm) which contains particularly high concentrations of glycogen (a polymer of glucose) and myoglobin (an oxygen-binding protein). The sarcoplasm contains a large number of mitochondria as well as some other highly specialised cytoplasmic organelles:

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Define the subdivision of the nervous system that motoneurones form a part of and be able to describe their structural features.

A

The contraction of skeletal muscle is under the control of the somatic nervous system which is part of the efferent (motor) division of the nervous system.

The nerve cells whose axons innervate skeletal muscle fibres are known as motoneurones. Their cell bodies are located either in the brainstem or in the anterior horns of the spinal cord. The axons of motoneurons are coated in a fatty material known as a myelin sheath, which is formed by specialised cells called Schwann cells. Motoneurons are the largest-diameter axons in the body. This allows them to conduct electrical signals (action potentials) at high velocities from the central nervous system to skeletal muscle fibres with minimum delay.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Appreciate the functional significance of the fact that motoneurones have myelinated axons.

A

The myelin sheath is formed during the early years of life, which partly explains why children need time to develop coordinated movement patterns. Individuals affected by certain neurological disorders, such as multiple sclerosis, experience degeneration of the myelin sheath and subsequent loss of coordination.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Describe the structure of the neuromuscular junction and know the functional significance of the major elements.

A

The myelin sheath ends near the surface of the muscle fibre, and the axon divides into a number of short ‘finger-like’ processes that lie embedded in grooves on the surface of the muscle fibre. The point of contact between axon terminals and the sarcolemma is referred to as the motor end plate. The junction between axon terminals and the motor endplate is known as the neuromuscular junction. In most cases each muscle fibre only receives a contact from one motoneurone. However, each motoneurone (as a consequence of its branching axon) can form a junction with more than one muscle fibre.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Define the term motor unit and understand the impact of motor unit size on the control of movement.

A

single motoneurone innervates many muscle fibres, but each muscle fibre is controlled by an axon terminal from only one motorneurone. One motoneurone together with all the muscle fibres it innervates is referred to as a motor unit.

Motor unit size varies quite significantly from muscle to muscle with the size being typically small in muscles involved in fine or precise movements and very large in muscles producing large forces requiring less precision.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Explain the mechanism by which an action potential in a motoneurone initiates the contraction of skeletal muscle fibres.

A

The process by which an action potential in a motoneurone initiates the contraction of all the muscle fibres that it innervates is referred to as neuromuscular transmission and involves a number of distinct stages:

  1. The action potential arrives at the end of the axon of the motoneurone and triggers the opening of the voltage-gated Ca2+ channels in the axon terminal.
  2. Ca2+ flows down its concentration gradient from the extracellular fluid into the axon terminal. Ca2+ then binds to proteins that enable synaptic vesicles to fuse with the neuronal plasma membrane, which subsequently results in the exocytosis of acetylcholine into the synaptic cleft which separates the axon terminal and the motor end plate.
  3. Acetylcholine then diffuses across the cleft and binds to the nicotinic acetylcholine receptors in the folds on the sarcolemma.
  4. The binding of acetylcholine to these receptors causes a depolarising graded potential in the muscle fibre which is known as the end-plate potential. The reason for this is that the binding of acetylcholine to this receptor triggers the opening of a relatively non-selective cation channel. This allows Na+ to flow into the cell and K+ out of the cell along their respective concentration gradients. However, the differences in electrochemical gradients that exists at the time of channel opening results in more Na+ flowing into the cell than K+ leaving the cell and so the overall effect is a net positive charge entering the cell and hence depolarisation.
  5. In most instances the magnitude of the endplate potential is 3-4 times greater than that required to reach the action potential threshold for the muscle fibre. Consequently in almost every instance, an action potential in the motoneurone produces an action potential in the muscle fibres it innervates
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Understand what is meant by an end-plate potential and how this results in action potential propagation along the length of muscle fibres.

A

The effect of acetylcholine at the neuromuscular junction is limited by two mechanisms. The first mechanism is simply the diffusion of acetylcholine away from the endplate. The second mechanism involves the degradation of acetylcholine by the acetylcholinesterases found in the cleft.

Note that in most respects the mechanism of neuromuscular transmission is very similar to that of an excitatory postsynaptic potential. However, the magnitude of the end plate potential is much greater than that of an excitatory postsynaptic potential because acetylcholine is released over a larger surface area, and binds to many more receptors, thereby opening many more ion channels. Therefore, one end plate potential is generally more than sufficient to depolarise the sarcolemma to its threshold potential, which results in an action potential.

Most neuromuscular junctions are located near the middle of muscle fibres. Newly generated action potentials produce local ion currents that depolarise adjacent regions, producing more actional potentials at the next site, and so on, to cause action potential propagation. Action potentials propogate from neuromuscular junctions across the surface of the muscle fibre in both directions towards the end of the fibre and throughout the T-tubule network. Note that inhibitory postsynaptic potentials do not occur in human skeletal muscle. All neuromuscular junctions are excitatory

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Describe the mechanism of excitation-contraction coupling.

A

The action potential produced at the end-plate (as a result of neuromuscular transmission) travels along the length of the muscle fibre (in both directions) with a conduction velocity of 1 to 5 m/sec. The mechanisms by which this muscle action potential triggers contraction of the muscle fibre is known as excitation-contraction coupling and involves a number of distinct steps:

A. The action potential travelling along the muscle fibre meets the T-tubules and flows down the T-tubules into the middle of the muscle fibre.

B. The presence of the action potential within the T-tubules triggers the opening of voltage-gated Ca2+ channels in the walls of the sarcoplasmic reticulum.

C. The opening of Ca2+ channels in the sarcoplasmic reticulum enables Ca2+ to run down its concentration gradient into the sarcoplasm and causes the sarcoplasmic Ca2+ concentration to increase from its resting level of 10-7M to around 10-4M.

D. The dramatic increase in sarcoplasm Ca2+ concentration triggers the molecular processes which cause the myofibril to shorten.

E. The myofibrils shorten virtually simultaneously which results in the contraction of the whole muscle fibre.

F. When action potentials stop flowing down the T-tubules, a calcium pump in the membrane of the sarcoplasmic reticulum pumps calcium out of the sarcoplasm and back into the sarcoplsmic reticulum.

G. The Ca2+ concentration within the sarcoplasmic reticulum then returns to resting levels, and myofibrils return to their normal length.

H. The muscle fibre relaxes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Understand the temporal relationship between an action potential, the tension generated and the sarcoplasmic Ca2+ levels in a single muscle fibre and be able describe how you could record these.

A

The temoral relationship between the events outlined above is illustrated in the diagram opposite. This shows the results of an experiment performed on a single isolated skeletal muscle fibre in which the muscle fibre action potential (blue) is recorded at the same time as the tension is measured (orange) and the sarcoplasmic Ca2+ concentration monitored (purple) using technique known as calcium imaging.

As you can see, there is a significant delay between the end of the action potential and the beginning of the contraction of the muscle fibre (as indicated by the increase in tension).

This delay reflects the time required for the Ca2+ channels to open, the Ca2+ to enter the sarcoplasm, and the molecular events which lead to myofibril shortening to be initiated. All this adds up to a significant delay between the action potential and the peak force being generated.

One initiated, the mechanical activity within the muscle fibre may last 100 ms or more. Similarly, there is a gradual decline in tension after the peak as the Ca2+ is pumped out of the sarcoplasm, and the myofibrils return to their resting length.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Describe the structure of thin myofilaments.

A

These filaments are in the order or 5-8 nm in diameter, around 1 micron long and are made up of three constituent proteins:

(i) Two chains of a globular protein molecules known as actin which are twisted around each other to form a double helix, much like two strands of pearls twisted together. Each actin molecule has a high affinity binding site for another protein known as myosin (see below). Actin forms the backbone of thin filaments.

actin
(ii) Two chains of the tube-shaped protein called tropomyosin which are wrapped around the actin helix and positioned in such a way that the myosin binding sites on each molecule of actin are blocked.

(iii) The troponin complex which hold the tropomyosin threads over the myosin binding sites on the actin molecules. Troponin has the ability to do this as a consequence of its three polypeptide units. One polypeptide binds to tropomyosin, another to actin and third which binds Ca2+ and plays a critical role in triggering contraction.

Collectively the actin double helix, the tropomyosin and the troponin complex interact to form a thin filament.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Describe the structure of thick myofilaments.

A

These thicker filaments are 12-18 nm in diameter and typically 1.6 microns in length. actinEach thick filament is made up of a large number of molecules of the protein myosin all packed together. Myosin makes up about two-thirds of all skeletal muscle protein. Each myosin filament typically is formed by about 200 myosin molecules. One end of each molecule of myosin is made up of two protein strands folded into a globular head, which looks a little like a golf club. If you can imagine the two shafts of the club wrapped around each other with the heads of the clubs sticking out of one end you will sort of get the picture.

The heads (referred to as cross-bridges) each have a binding site for actin (complementary to the myosin binding site on the actin molecules) and an ATPase. A few hundred of these myosin molecules lie parallel to each other with their shafts forming the filament itself and the radiating heads forming the cross-bridges.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Explain how thick and thin myofilamants are arranged and why this results in the typically striated appearance of skeletal muscle.

A

So the two basic building blocks of the myofibril are these thick and thin filaments, but how do these relatively short proteins interact to form the myofibrils which may run the length of the muscle fibre? The answer to this underlies one of the most striking features of skeletal muscle fibres under the microscope and that is that they have a distinctive striped or ‘striated’ appearance.

The striations observed in the whole muscle fibre represent alternating dark and light bands along the length of myofibrils. Because all the myofibrils in a muscle fibre are similarly aligned, when you look at a skeletal muscle fibre it appears striated. The dark bands are known as A bands, the light bands as I bands and the thin line which is sometimes observed down the middle of the I bands is known as the Z line.

The diagram opposite shows part of a myofibril illustrating the alternating dark and light bands and the nomenclature used to identify the different bands. Also shown is the arrangement of the thick (pink) and thin (blue) filaments that gives rise to the striations.

The alternating dark and light bands are caused by the highly ordered arrangement of the thin and thick filaments within the myofibril. The thick filaments are surrounded by six thin filaments in a hexagonal arrangement.

The dark A-bands represent the regions that contain both the very much denser thick filaments and the less dense thin filaments. The light I-bands represent the regions that contain thin filaments only.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Explain what a sarcomere is and how the sarcomere length changes during muscle contraction and relaxation.

A

The Z line represents the junctions between successive blocks of thin filaments, and indicates the boundaries of the basic functional unit of a myofibril which is known as a sarcomere. Essentially, whatever happens between the two Z lines is simply repeated again and again along the length of the entire myofibril. Consequently, by observing the changes that take place between two Z lines, we can see what is happening to the entire myofibril.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Describe the molecular interactions between thick and thin filaments that occur during the five distinct stages of the sliding-filament mechanism of muscle contraction.

A

When a muscle fiber contracts, the overlapping thick and thin filaments in each sarcomere ‘slide’ past each other, propelled by movements of the myosin cross-bridges.

During this shortening of the sarcomeres, there is actually no change in the lengths of either the thick or thin filaments.

Rather, as a result of the filaments sliding past each other, the entire sarcomere shortens in length. Typically duringmuscle movement, one end of the muscle remains at a fixed position, while the other shortens toward the fixed position.

As for excitation-contraction coupling, we will consider the sliding-filament mechanism in a number of distinct stages. Use the key below to identify all the elements in the subsequent figures.

A. Rest:
Prior to any action potential arriving in the muscle fibre the sarcoplasmic Ca2+ concentration is very low. Tropomyosin molecules cover the myosin-binding sites on the actin molecules, thereby preventing the myosin cross-bridges from binding to actin.

The cross-bridges are, however, in an energised or ‘primed’ state as a result of the splitting of ATP molecules. The products of this reaction (ADP and inorganic phosphate) remain bound to the myosin cross-bridges.

This energy storage in myosin is analogous to the storage of potential chemical energy in a stretched spring (i.e., energy has been expended in stretching the spring and remains stored in the spring).

B. Binding:

As a result of the release of calcium from the sarcoplasmic reticulum, the sarcoplasmic Ca2+concentration increases to 10-4M and some of this Ca2+ binds to the calcium binding sites on the troponin complex.

This binding changes the shape of the troponin complex, which in turn displaces the tropomyosin molecules from the myosin-binding sites on the actin molecules.

This process then allows the myosin cross-bridges to bind to the thin filaments. The thick and thin filaments are now coupled.

C. Power Stroke:

The myosin cross-bridge swings over, and binds weakly to a new actin molecule, and is now at an ange of 90 degree relative to the thin filaments. Inorganic phosphate released in this process initiates the ‘power stroke’, whereby the myosin cross-bridge rotates on its hinge, pushing the actin filament past it.

The net effect of this step is that the sarcomere length (the distance between two Z-lines) has decreased because the same process is repeated on both ends of the thick filament.

At the end of this stage the ADP (which is still bound to the ATPase) is released.

D. Detachment:

During the cross-bridge movement, myosin is bound very firmly to actin. This linkage must be broken to allow the cross-bridge to be primed again and repeat the cycle.

The release of the ADP from the ATPase allows a new ATP molecule to bind to myosin, which in turn breaks the link between actin and myosin, allowing them to separate.

If there is still Ca2+ in the sarcoplasm then the tropomyosin filament will still be displaced, the cross bridges are able to bind again to the thin filaments, and the whole cycle is repeated again.

E. Relaxation:

However if there is no Ca2+ available for binding, the troponin complex will return to its original shape and the tropomyosin filaments will resume their blocking position over the myosin binding sites on the thin filaments.

As a consequence, the thick and thin filaments are prevented from binding and will simply slide past each other resulting in the sarcomere length increasing. Note that this means that whereas the contraction of the myofibril requires energy, the relaxation of the myofibril is passive (i.e., does not require energy).

The animation on the right shows ONE complete cross-bridge cycle which begins with the influx of Ca2+ and finishes with the cross-bridge being “primed” again by the break down of ATP. In reality, each cycle only decreases the sarcomere length by approximately 20 nm (around 1% of its total length), whereas a single action potential causes a sarcomere to shorten by around 40%. Obviously this means that the cross-bridge cycle is repeated many times following just a single action potential.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Know what rigor mortis is and be able to explain its molecular basis.

A

Rigor mortis is the stiffness which skeletal muscles begin to exhibit about four hours after death, reaches a peak after about 12 hours, and subsides after about 2-3 days.

The stiffness is thought to be due to the leakage of Ca2+ into the sarcoplasm from both the extracellular fluid and the sarcoplasmic reticulum, as the cells die and are unable to maintain the rather large differences in ion concentrations which are present in these compartments during life.

Some of this calcium binds to the troponin complex, displaces the tropomyosin filament, allows binding of the cross bridges and initiates the power stroke which results in the muscle fibres shortening.

However you will recall from the description of the sliding-filament mechanism that at this stage ATP is required to bind to the cross bridges to detach the thick filaments from the thin filaments.

Of course when you are dead there isn’t any ATP lying around, and so the muscle fibres remain in this contracted state until the molecular structure of the filaments starts to decompose. Not very pleasant I guess but it does highlight (1) the importance of Ca2+ in triggering the process, (2) the fact that the cross bridges are primed before cross-bridge binding occurs and (3) that ATP is required for cross bridge detachment.

17
Q

Understand why skeletal muscle is sometimes referred to as voluntary muscle and the difference between isotonic and isometric contractions.

A

Skeletal muscle is of course also known as voluntary muscle because it is directly under the control of motoneurones. In addition to this neural regulation, however, the mechanics of the muscle itself plays an important role in the control over the force generated. In this section we will take a fairly brief look at some of the factors (both neural and mechanical) which act to modify the force of skeletal muscle contraction.

First of all we need to appreciate that skeletal muscles are actually capable of two major types of contraction, as described below. Note that the term contraction in the context of muscle physiology does not necessarily mean ‘shortening’. Rather, it simply refers to activation of the contractile machinery of muscle fibres - the cross-bridges. Following contraction, this contractile machinery is switched off, and tension declines, allowing the muscle fibre to relax.

Isotonic Contraction: This is where the tension the muscle remains constant as the muscle shortens. This is the type of contraction that occurs (for example) when you lift a glass off and move it towards your mouth. The mass of the glass doesn’t change during the movement (so the tension in your muscle doesn’t change) BUT the length of the biceps brachii muscle decreases as you flex your forearm. Because the tension remains the same, the contraction is considered to be isotonic. This is the type of contraction which actually produces movement.

Isometric Contraction: This is where the contraction of the muscle produces tension but the muscle’s length does not change. An example of this type of contraction is when you put your hand under a fixed bench (palm up) and try to lift it. Your biceps brachii is generating plenty of tension but the length of the muscle remains the same (hence the name isometric). This is the type of contraction you use when you want to hold something still, and is also vital for the maintenance of posture.Isometric contractions are also sometimes referred to as static contractions.

It is important to realise that the mechanisms for producing isometric and isotonic contractions are exactly the same (i.e. cross-bridge interactions between the myofilaments). The only difference is that isotonic contractions involve sarcomere shortening whereas isometric contractions don’t. There are in fact a large number of factors which regulate the force of contraction produced by a skeletal muscle. Time does not permit an exhaustive consideration of these here but we will deal with three of the more physiologically important ones.

18
Q

Understand how the principle of orderly recruitment regulates the force of contraction generated by a skeletal muscle.

A

You will recall from a previous section that a motor unit consists of a single motoneurone plus all the muscle fibres that it innervates. Consequently for a very weak contraction of the muscle, only a few motor units are recruited (activated) whereas more and more motor units are recruited when progressively larger contractions are required. This is known as the principle of orderly recruitment.

Interestingly, during sustained contractions of skeletal muscles (e.g. when you hold your arm out in front of you for a long time) the motor units take turns at doing the work. This progressive change in motor unit recruitment allows some motor units to rest whilst others continue doing to work. This is of course all carefully regulated by the neural circuits within the central nervous system that control movement.

A mechanism that may partially explain the principle of orderly recruitment is the size principle, which states that the order of recruitment of motor units is directly related to the size of their motoneurone. Motor units with smaller motoneurones will be recruited first.

19
Q

Explain how action potential frequency is involved in regulating skeletal muscle contraction and how temporal summation contributes to this process.

A

Another way that the tension produced by a skeletal muscle fibre can be increased is by increasing the action potential frequency in the motor units. In the same way that excitatory post synaptic potentials exhibit temporal summation at synapses, so too can the force generated by action potentials in skeletal muscle fibres. This is possible because of the relatively long time that the tension generated by a single action potential lasts (up to 100 ms).

The diagram opposite shows that the tension (red) generated in a skeletal muscle fibre in response to a single action potential (blue) last much longer than the action potential. The smallest contractile response of a muscle fibre or a motor unit to a single electrical stimulus is termed a twitch.

When the action potentials in the muscle fibre are sufficiently far apart (in time) the tension produced by the second action potential is the same as the first.

However, if the time between the two action potentials is decreased (you can simulate this by clicking on the +/- buttons below) then the tension produced by the second action potential increases before the tension produced by the first has declined and so we get temporal summation resulting in increased tension.

If the action potential frequency is increased then you observe a progressive increase in the force generated due to this summation. Eventually increasing the action potential frequency even more produces no further increase in the tension produced. This is referred as a tetanic contraction

20
Q

Describe an experiment that you could before to demonstrate the principle of temporal summation in skeletal muscle.

A

Another very important factor which alters the amount of force a muscle can generate is the length of the muscle prior to contraction.

There is an optimal length of each muscle fibre relative to its ability to generate force. Recall that a muscle fibre is comprised of sarcomeres connected end to end and that these sarcomeres are composed of both thick and thin filaments.

The optimal sarcomere length (and therefore muscle length) is that length where there is optimal overlap of the thick and thin filaments, thus maximising cross-bridge interaction. This can be demonstrated fairly easily in an experiment where you measure the force generated during a tetanic contraction of a single muscle at different starting lengths.

The diagram opposite shows the apparatus used to investigate the length-tension relationship in a skeletal muscle. The muscle is attached to a micromanipulator which allows the length of the muscle to be varied. The nerve supplying the muscle is stimulated at a frequency that which produces a tetanic contraction and the force generated during this contraction is recorded using a tension gauge.

21
Q

Know the difference between a twitch and tetanic contraction in skeletal muscle.

A

The diagram opposite shows that the tension (red) generated in a skeletal muscle fibre in response to a single action potential (blue) last much longer than the action potential. The smallest contractile response of a muscle fibre or a motor unit to a single electrical stimulus is termed a twitch.

If the action potential frequency is increased then you observe a progressive increase in the force generated due to this summation. Eventually increasing the action potential frequency even more produces no further increase in the tension produced. This is referred as a tetanic contraction.

22
Q

Understand what is meant by the length-tension relationship and how you could demonstrate this experimentally.

A

The diagram opposite shows the apparatus used to investigate the length-tension relationship in a skeletal muscle. The muscle is attached to a micromanipulator which allows the length of the muscle to be varied. The nerve supplying the muscle is stimulated at a frequency that which produces a tetanic contraction and the force generated during this contraction is recorded using a tension gauge.

The type of result you get when you plot starting length against tetanic tension in this type of experiment is shown opposite. As you can see, the maximal force which can be generated is around the the normal resting length of the muscle (point B on the graph) but that it drops off very quickly if the muscle is too short (point A) OR stretched (point C) prior to stimulation.

23
Q

Explain the physiological-basis of the length-tension relationship.

A

One explanation for this is illustrated by the cartoons on the right that illustrate the postulated appearance of a sarcomere at these three different points on the length-tension relationship.

At the normal resting range (B) there is a maximum overlap between the thin and thick filaments so the maximal tensin can be generated.

However when the muscle is very short the thin filaments overlap (A) whilst stretching the muscle only permits interactions between the very ends of the thick filaments and the thin filaments (C). In both instances, the opportunities for cross-bridge interactions are greatly reduced, and consequently so is the force that can be generated.

This rather neat explanation remains fairly speculative, but it does support the sliding-filament mechanism on one hand, and also explains (to some degree) why the most difficult phase of a pull-up is the initial 2-3 cm of movement (from a dead-hang) (i.e., when the muscles are at their longest length) and the phase where you have to get your chin over that bar (i.e., when the muscles are at their shortest length).

24
Q

Describe the difference in physiological properties of the two major types of skeletal muscle fibre and the implication of this on athletic performance.

A

There are two major types of skeletal muscle fibres classified originally upon the rate at which they developed force when contracting (as shown in the diagram opposite):

Slow-Twitch (Type I) Fibres
As their name suggests, these fibres develop force at a comparatively slow rate. They are typically smaller in diameter, have a rich capillary network surrounding them and have particularly high concentrations of myoglobin (an oxygen-binding protein) in their sarcoplasm.

These types of fibres appear to be fairly resistant to fatigue and particularly well suited to movements requiring low force but sustained contractions (e.g. distance running).

Fast-Twitch (Type II) Fibres
These fibres are significantly larger in diameter and develop force roughly three times faster that slow-twitch fibres. Some fast-twitch fibres are fairly resistant to fatigue and have a fairly high myoglobin concentration (and are known as Type IIa fibres) whilst other are more easily fatigable and have lower concentrations of myoglobin (Type IIb fibres).

Type II fibres are better suited for short duration, high force type activities (e.g. sprinting or pole-vaulting).

In humans, individual muscles contain a mix of slow-twitch and fast-twitch muscle fibres, so what determines when these different muscle fibres are recruited? You will recall that a motor unit consists of a motoneurone together with all the muscle fibres that it innervates. Well, it turns out that the muscle fibres that form a motor unit are always of the same type. Consequently, the nervous system can select to activate slow-twitch or fast-twitch fibres (or even their sub-types), simply by activating the appropriate motoneurone.

25
Q

Explain the theory behind electromyography.

A

EMG signals are recorded by placing electrodes close to the muscle groups. When the muscle is activated, the length of the muscle decreases and the muscle, skin and electrodes move with respect to one another. At that time, the electrodes will show some movement artifacts

26
Q

Demonstrate how to record an electromyogram and carry out qualitative and quantitative analyses of the recording.

A

During a needle EMG, a needle electrode inserted directly into a muscle records the electrical activity in that muscle. A nerve conduction study, another part of an EMG, uses electrode stickers applied to the skin (surface electrodes) to measure the speed and strength of signals traveling between two or more points.

27
Q

Understand the uses of electromyography in sport and clinical sciences.

A

EMG allows clinicians to study muscular physiology and diagnose neuromuscular diseases (Figure 1). Furthermore, EMG can be used to detect spasms, muscular hyperactivity, muscular imbalances, and the resting and occlusal positions of the jaw

28
Q

Know the major functions of muscle and appreciate that there are three different types of muscle

A

Muscles allow a person to move, speak, and chew. They control heartbeat, breathing, and digestion. Other seemingly unrelated functions, including temperature regulation and vision, also rely on the muscular system. Keep reading to discover much more about the muscular system and how it controls the body

Three muscle types:

  • skeletal
  • smooth
  • cardiac
29
Q

Describe the functional significance of myofibrils, the sarcoplasmic reticulum and transverse (or T-) tubules.

Myofibrils:

A

Myofibrils:
Each muscle fibre contains a large number of cylindrical bundles, approximately 1-2 micrometres in diameter. These myofibrils occupy approximately 80% of the volume of the sarcoplasm and run the entire length of the muscle fibre. Myofibrils are functionally important as they contain the myofilaments which comprise the contractile elements of the muscle fibre.

30
Q

Describe the functional significance of myofibrils, the sarcoplasmic reticulum and transverse (or T-) tubules.

Sarcoplasmic reticulum:

A

Each myofibril is surrounded by a series of ‘sleeve-like’ segments. At the end each segment are two enlarged regions known as lateral sacs, which are connected to each other by a series of smaller tubular elements. Sarcoplasmic reticulum serves as a storage site for calcium, which is essential for muscle contraction. In a typical skeletal muscle fibre, the sarcoplasmic reticulum contains around 10-3M Ca2+ which is significantly higher than the 10-7M Ca2+present in the sarcoplasm at rest. As we will see, the close relationship between the myofibrils and the sarcoplasmic reticulum as well as the high concentration of Ca2+ in the latter plays an important role in the control of muscle contraction

31
Q

Describe the functional significance of myofibrils, the sarcoplasmic reticulum and transverse (or T-) tubules.

Transverse (or T-) Tubules:

A

T-tubules are located at regular intervals along the length of muscle fibres. They are located directly between, and are closely linked with the lateral sacs of adjacent segments of the sarcoplasmic reticulum. The arrangement of these tubules is such that their lumen is continuous with the extracellular space. T-tubules play a very important role in the initiation of muscle fibre contraction by conducting electrical signals from the sarcolemma into the interior of the muscle fibre.

32
Q

Each myofibril is made up of many thousand smaller filamentous structures of two major types:

A

Thin filaments and thick filaments

33
Q

Explain which fibres are utilised for various activites/sports

A

Which skeletal muscle fibre types are activated depends entirely upon the type of activity being undertaken:

Slow-twitch fibres are recruited when the muscle is required to produce any force at all but are fairly limited in the maximal force that they can generate.
Type IIa fast-twitch fibres are recruited when intermediate forces are required.
Type IIb fast-twitch fibres are recruited when higher intensity activities are undertaken that require even more force to be generated.

Given the functional diversity of skeletal muscle fibre types it is not surprising that exercise physiologists have considerable interest in the relative number of these fibre types in different athletes.

Clearly muscles that have a higher proportion of fast-twitch fibres will produce more power than muscles containing predominantly slow-twitch fibres. Such muscles would be particularly suited to high-intensity (power) sports.

On the other hand, muscles with a predominantly slow-twitch fibre type would be more efficient in endurance sports.

As shown in the table on the right, analysis of the relative proportion of slow-twitch and fast-twitch fibre types in elite athletes has revealed a predominance of slow-twitch fibres in endurance runners and fast-twitch fibres in sprinters.