Lactate Dehydrogenase

Question 1

Lactate dehydrogenase has —- subunits

Answer 1

4

Question 2

The subunits May be either

Answer 2

Heart H

M muscle

Question 3

The combinations of H and M are

Answer 3

LDH-1 H4
LDH-2 H3M1
            H2M2
            H1M3
            M4

Question 4

The isoenzymes are separated by ———— at pH ——

Answer 4

Cellulose acetate electrophoresis, 8.6

Question 5

LDH converts ——— to lactate

Answer 5

Pyruvate

Question 6

Normal value of LDH in serum is

Answer 6

100-200U/L

Question 7

M4 is seen in

Answer 7

Skeletal muscles

Question 8

H4 is seen in

Answer 8

Heart

Question 9

In myocardial infarction, what is flipped pattern

Answer 9

Normally LDH 2 concentration in blood is greater than LDH-1 but this is reversed in myocardial infarction

Question 10

LDH will increase ——— hr after MI, reach a Peak by ——- hrs and the level will go back to original by ———- days

Answer 10

6-12 hrs
24-48hrs
6-8 days

Question 11

LDH level is ———- times more in the RBC than in plasma

Answer 11

100

Question 12

Minor amount of hemolysis will result in a false positive test
True or false

Answer 12

True

Question 13

Electrophoretic mobility of —— is fastest

Answer 13

LDH 1

Question 14

Electrophoretic mobility of ——- is slowest

Answer 14

LDH-5

Question 15

LDH —- and —— are not destroyed at high temp

Answer 15

1 and 2

Question 16

LDH - and - are destroyed at high temp

Answer 16

4 and 5

Question 17

LDH 2 is produced by

Answer 17

RBC brain

Question 18

LDH 3 is produced by

Answer 18

Brain and leukocytes

Question 19

LDH 4 is produced by

Answer 19

Liver and leuokocytes

Question 20

Percentage in human serum is 35%

Answer 20

LDH2

Question 21

LDH 5 level is increased in

Answer 21

Prostate

Breast carcinoma

Question 22

Km value increases for ——— and decreases for ———

Answer 22

LDH1

LDH5