Alkaline Phosphatase

Question 1

The pH optimum for enzyme reaction of alkaline phosphatase

Answer 1

9-10

Question 2

ALP is activated by

Answer 2

Magnesium and manganese

Question 3

ALP contains

Answer 3

Zinc

Question 4

Osteoblasts of bone (calcification process) produce

Answer 4

ALP

Question 5

An ectoenzyme localized in cell membrane is

Answer 5

ALP

Question 6

Serum value of ALP is

Answer 6

40-125 U/L

Question 7

Moderate increase in ALP is seen in (2-3 times)

Answer 7

Hepatic diseases
Infective hepatitis
Alcoholic hepatitis
Hepatocellular carcinoma

Question 8

Very high levels of ALP (10-12 times) are seen in

Answer 8

Extrahepatic obstruction
Obstructive jaundice 
Gallstones
Pressure on bile duct
Carcinoma of head of pancreas

Question 9

Intrahepatic cholestasis is due to

Answer 9

Virus (infective hepatitis)

Drugs (chlorpromazine)

Question 10

ALP is produced by

Answer 10

Epithelial cells of biliary canaliculi

Question 11

Drastically high levels of ALP (10-25 times) are seen in

Answer 11

Bone diseases 
Paget’s disease (osteitis déformans)
Rickets
Osteomalacia
Osteoblastoma
Metastatic carcinoma of bone
Hyper parathyroidism

Question 12

Alpha-1 ALP is synthesized by

Answer 12

Epithelial cells of biliary canaliculi

Question 13

Alpha 1 ALP is increased in

Answer 13

Obstructive jaundice

Question 14

Alpha 2 heat labile ALP is stable at —— but loses its activity when kept at ————— for 30 min

Answer 14

56C. 65C

Question 15

Alpha 2 heat stable ALP will not be destroyed at ——— but is inhibited by ————-

Answer 15

65C phenylalanine

Question 16

Alpha 2 heat stable ALP is of ————- origin

Answer 16

Placental

Question 17

An isoenzyme resembling placental form which is seen in circulation in carcinoma of lung liver and gut is

Answer 17

Regan isoenzyme

Question 18

Pre beta ALP is of —- origin

Answer 18

Bone

Question 19

Pre beta ALP is destroyed at —-

Answer 19

56C heat lablile

Question 20

50% of ALP activity is constituted by

Answer 20

Pre beta ALP