Laboratory techniques

Question 1

Laboratory procedure used to amplify a desired fragment of DNA

Answer 1

PCR

Question 2

Blotting procedure used to analyze DNA

Answer 2

Southern blot

Question 3

Blotting procedure that identifies DNA-binding proteins like transcription factors

Answer 3

Southwestern blot

Question 4

Blotting procedure used to analyze RNA

Answer 4

Northern blot

Question 5

Confirmatory test for HIV after positive ELISA is what type of blotting procedure

Answer 5

Western blot

Question 6

Blotting procedure useful for studying mRNA levels

Answer 6

Northern blot

Question 7

Blotting procedure used to analyze proteins

Answer 7

Western blot

Question 8

Lab technique used to assess size, granularity, and protein expression of individual cells in a sample

Answer 8

Flow cytometry

Question 9

Used to profile gene expression levels of multiple genes simultaneously to study diseases and treatments

Answer 9

Microarrays

Question 10

Commonly used in workup of hematologic abnormalities

Answer 10

Flow cytometry

Question 11

Lab technique use to detect single nucleotide polymorphisms (SNPs) and copy number variations (CNVs)

Answer 11

Microarrays

Question 12

Immunologic test used to detect the presence of either a specific antigen or antibody in a patient’s blood sample

Answer 12

Enzyme-linked immunosorbent assay

Question 13

Enzyme-linked immunosorbent assay detection involves the use of what?

Answer 13

Antibody linked to an enzyme

Question 14

What produces a detectable signal in Enzyme-linked immunosorbent assay

Answer 14

Added substrate reacting with enzyme

Question 15

Lab technique used to diagnose chromosomal imbalances

Answer 15

Karyotyping

Question 16

Lab technique used for specific localization of genes and direct visualization of chromosomal anomalies at the molecular level

Answer 16

Fluorescence in situ hybridization

Question 17

Chromosome abnormality with fluorescence signal that corresponds to one chromosome is found in a different chromosome

Answer 17

Translocation

Question 18

Chromosome abnormality with extra site of fluorescence on one chromosome relative to its homologous chromosome

Answer 18

Duplication

Question 19

Chromosome abnormality with no fluorescence on a chromosome compared to fluorescence at the same locus on the second copy of that chromosome

Answer 19

Microdeletion

Question 20

Process causing a recombinant DNA molecule to self-perpetuate

Answer 20

Cloning

Question 21

Unique property of cDNA

Answer 21

Lacks introns

Question 22

Function of RNA interference

Answer 22

Promote degradation of target mRNA

Question 23

Cre-lox system

Answer 23

Manipulates genes at specific developmental points