Laboratory Techniques

Question 1

Quadrant Streaking Technique

Answer 1

Reduces or dilutes the number of cells using successive streaking zones on the petri plate and flaming the inoculation loop between zones

Question 2

Motivation

Answer 2

The energizing of behavior in pursuit of a goal

Question 3

Dilution Formula

Answer 3

(M1)(V1) = (M2)(V2)

Question 4

Technological vs Biological Replicates

Answer 4

Technological: Same sample, different readings

Biological: Different samples

Question 5

UAS - GAL 4 System

Answer 5

A binary expression system

GAL 4 comes from the yeast genome and is inserted into dorsophila and is regulated by an enhancer

In a separate fly, there is a UAS (Upstream activation sequence) that when GAL 4 is bound to it, expresses a reporter gene

The flies are crossed resulting in a fly with UAS - GAL 4 that has the ability to only produce GAL 4 in specific tissues allowing the reporter gene to be expressed

Question 6

Binary Expression System

Answer 6

Any system that allows for the over expression or “knocking out” of a gene

Question 7

RNAi

Answer 7

Used for gene regulation, RNAi will bind to a single strand of RNA forming double stranded RNA which will be destroyed

Question 8

Hebbian learning

Answer 8

Neurons that fire together wire together so as the pre synaptic neuron fires and stimulates the post synaptic neuron the synapse improves.

Question 9

En passant Synapse

Answer 9

In contrast to a normal neural synapse an En Passant synapse forms along the length of the axon and is usually only a temporary linkage

Question 10

Start Codon

Answer 10

A codon that is recognzied by a ribosome to start translation of a genetic sequence

Question 11

Stop Codon

Answer 11

Another specific codon that is recognized by the ribosome to stop translation.