Laboratory Safety Guidelines Flashcards
Before the actual experiment, what must you do?
Prepare a work plan
What does a work plan contain?
(familiarize) objectives, instructions and procedures
What must you wear to ensure personal safety in the laboratory?
Prescribed Personnel Protective Equipment
What must be worn during experiments?
Safety goggles
Closed shoes (at all times)
Prescribed Laboratory Gown
Protective Face mask (recommended)
As an additional safety measure, ________ should be properly secured to avoid interference in your work.
long hair and loose clothing
What should you immediately report to your laboratory instructor?
any major spills, breakages, accidents
all physical injuries sustained in the lab (no matter how trivial)
all “near-miss” incidents
The students must wear PPE to enter the laboratory and carry out an experiment
What must you avoid to wear in the lab?
Avoid wearing jewelry
What is strictly prohibited in the laboratory? Why?
Eating, drinking and chewing gum
Most chemicals in the laboratory are poisonous
Why is smoking prohibited in the laboratory?
Some of the laboratory chemicals are flammable
Flammable solvents like organic solvents must be only used under the ____________
fume hood
Perform experiments only during the _________ and only under the supervision of your ____________
scheduled laboratory period, laboratory instructor.
Never work alone
Only __________ experiments must be carried out during your laboratory period. Moreover, no _____________ from the experiment procedures described in this manual, is allowed without the permission of the laboratory instructor.
scheduled, additional experiment procedures or deviations
DO NOT PERFORM UNAUTHORIZED EXPERIMENTS.
NEVER REMOVE CHEMICALS FROM THE LABORATORY AND STOCKROOM - why?
Improper handling and misuse of these chemicals could pose great health and environmental hazard.
Do not play games or engage in horseplay in the laboratory. Concentrate on performing your experiment.
NO LOITERING IN THE LABORATORY
DO NOT LEAVE AN ON-GOING EXPERIMENT SET-UP *.
UNATTENDED
Carefully read the * of chemical reagent containers – check the *
labels, formula and the concentration.
Examine the * and know where to find specific information on the hazards, toxicology, immediate first aid, and correct disposal of the substance listed on it
Material Safety Data Sheet (MSDS)
Know the hazards associated with the chemicals as well as their physical and chemical properties.
HANDLE ALL CHEMICALS WITH EXTREME CAUTION.
Most chemicals are * . Exercise caution when handling * acids and * .
corrosive
concentrated, ammonia
Concentrated acids must only be used under the *
fume hood.
In case of a chemical spillage, immediately take off any * clothing and wash off spilled chemicals on the skin or eyes with copious running water for at least +
contaminated
15 minutes.
Take extra care in handling chemicals in the form of * . It is recommended that you wear a mask to avoid inhalation of these chemicals. If these come into contact with the eyes, wash with copious running water continuously for at least 15 minutes. Consult a physician if needed.
fine dust or powder
Observe * when working in the laboratory.
cleanliness and orderliness
- in the laboratory is a must
Cleanliness
- Avoid * the reagents.
2. Practice the habit of * droppers and rods immediately after use.
contaminating
rinsing
- Refrain from accumulating dirty glassware. Use * (e.g. while heating solutions) to clean glassware.
- Always wash or wipe off * from reagent bottles.
waiting times
dirt
- Clean up * immediately even if it is only water.
6. * before leaving the laboratory and dry them on a clean towel.
spills, water
Wash your hands thoroughly
Dispose acids and bases into *, respectively, to prevent clogging of sinks or premature corrosion of pipes.
acid waste container and base waste container
Excess and waste potassium permanganate (KMnO4) should also be thrown into * containers since this is a powerful oxidant that may corrode sink pipes.
inorganic waste
Excess and waste * should also be thrown into inorganic waste containers since this is a powerful oxidant that may corrode sink pipes.
potassium permanganate (KMnO4)
Dispose organic wastes into *
halogenated organic waste containers or non-halogenated organic waste container.
All solids used in experiment procedures (filtration, chromatography, etc.) should be disposed into *
contaminated solid waste containers.
Other solid wastes should be disposed into the * available inside the laboratory.
trash bin
All broken glassware including * used in experimental procedures such as melting point determination, chromatography, etc. should be disposed in *
capillary tubes, broken glassware container.
What are the 4 types of waste and where should they be disposed of?
Inorganic waste (acids and bases + Potassium permanganate (KMnO4) Organic waste (non/halogenated organic waste containers Solid waste (contaminated solid waste containers; trash bin) Broken glassware (like capillary tubes - broken glassware container)
Flammable organic solvents
ethanol, methanol, acetone
Since there are few flammable organic solvents, a chemical fire can occur. Also, there is a small risk of electrical fire.
In case a fire occurs, what is the first thing you should do?
Remain calm (Call the attention of your laboratory instructor and leave the laboratory)
Fires in small containers can be extinguished by * over the burning container. Alternatively, cover the burning container with *. Never use * towels.
inverting a small beaker or any other similar equipment
soaking wet towels
dry
Remove any flammable material such as * from the area of the fire.
reagent bottles with flammable chemical
In case of a person inhaling smoke or toxic fumes, transfer him/her to a *.
well-ventilated area
In case your clothing should catch on fire, use the *
safety shower
