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BACTERIOLOGY P1 > LABORATORY SAFETY AND INFECTION CONTROL > Flashcards

LABORATORY SAFETY AND INFECTION CONTROL Flashcards

1
Q

used for dangerous chemicals

A

Chemical fume hood

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2
Q

protect the users

A

chemical fume hood

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3
Q

no hepa filter

A

chemical fume hood

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4
Q

exhaust air outside the building

A

chemical fume hood

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5
Q

used for infectious biological agents, protects the user, the environment and the material

A

Biosafety cabinet

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6
Q

possess HEPA filter does not exhaust air outside the building without decontamination

A

Biosafety cabinet

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7
Q

HEPA

A

HIGH EFFICIENCY PARTICULATE AIR

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8
Q

A device that encloses a working area
to protect workers from aerosol
exposure and infectious disease
agents.

A

Biosafety cabinet

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9
Q

the air that contains the
infectious materials is sterilized, either
by heat, UV light or passage through a
high-efficiency particulate (HEPA)
resistance filter.

A

BSC

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10
Q

Open-fronted type of cabinet with
negative pressure (ventilated
cabinets)

A

CLASS I CABINET

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11
Q

It allows room (unsterilized) air to
enter the cabinet, circulate around
the area and expose the material
within; only the air to be exhausted
is sterilized using a HEPA filter.

A

CLASS I CABINET

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12
Q

It is used for biosafety levels 2 and 3
agents

A

CLASS I CABINET

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13
Q

It is also known as the laminar flow BSC

A

CLASS II CABINET

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14
Q

The most commonly used BSC in a clinical
microbiology laboratory.

A

Class IIA

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15
Q

Sterilizes the air using HEPA filter that flows over the infectious material and the air to be exhausted.

A

CLASS II CABINET

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16
Q

has fixed opening; 70% of the air is recirculated

A

Class IIA

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17
Q

variable sash opening; used for chemicals, radioisotopes and carcinogens.

A

Class IIB

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18
Q

Provides the highest level of
safety to the worker

A

CLASS III CABINET

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19
Q

The air coming into and going out
of the cabinet is sterilized using
HEPA filter and the infectious
material within is handled with
rubber gloves that are attached
and sealed in the cabinet.

A

CLASS III CABINET

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20
Q

It is used for BSL 4 agents.

A

CLASS III CABINET

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21
Q

Saccharomyces cerevisiae, E. coli K-12, and non-infectious bacteria

A

BSL-1

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22
Q

Hepatitis a virus, Streptococcus pyogenes, Borrelia burgdorferi (Lyme disease), Salmonella species

A

BSL-2

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23
Q

Yersinia pestis (plague), Mycobacterium tuberculosis, SARS, rabies virus, West Nile Virus, hanta viruses

A

BSL-3

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24
Q

Ebola virus, smallpox virus

A

BSL-4

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25
Q

Not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment.

A

BSL-1

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26
Q

Moderate potential hazard to personnel and the environment. Includes bacteria and viruses that cause mild disease to humans, or are difficult to contract via aerosol in a lab setting.

A

BSL-2

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27
Q

Microbes there can either indigenous or exotic, and they can cause serious or potentially lethal disease through respiratory transmission.

A

BSL-3

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28
Q

Dangerous and exotic, posing a high risk of aerosol-transmitted infections. Infections caused by these microbes are frequently fatal and without treatment or vaccines.

A

BSL-4

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28
Q
  • No Containment
  • Defined organisms
    -Unlikely to cause disease
29
Q
  • Containment
  • Moderate Risk
  • Disease of varying severity
30
Q
  • High Containment
  • Aerosol Transmission
  • Serious/ Potentially lethal disease
31
Q
  • Max Containment
  • “Exotic”, High- Risk Agents
  • Life- threatening disease
32
Q

E. coli

33
Q

Influenza, HIV, Lyme Disease

34
Q

Tuberculosis

35
Q

Ebola Virus

36
Q

Agents that present minimal potential hazard to personnel & the environment.

37
Q

Agents associated with human disease & pose moderate hazards to personnel & the environment.

38
Q

Indigenous or exotic agents, agents that present a potential for aerosol transmission, & agents causing serious or potentially lethal disease.

39
Q

Dangerous & exotic agents that pose a high risk of aerosol-transmitted laboratory infections & life-threatening disease.

40
Q

Pass-thru autoclave with Bioseal required in laboratory room.

41
Q

Pass-thru autoclave with Bioseal required in laboratory room.

42
Q

These are agents that pose the greatest public health threat.

A

Category A Agents

43
Q

They are easily transmitted and highly infectious.

A

Category A Agents

44
Q

Smallpox, Bacillus anthracis, and Francisella tularensis

A

Category A Agents

45
Q

These are agents with moderate morbidity and low mortality.

A

Category B Agents

46
Q

Not easily transmitted as category A agents.

A

Category B Agents

47
Q

Coxiella burnetti, Burkholderia pseudomallei, and Rickettsia

A

Category B Agents

48
Q

These are the emerging pathogens

A

Category C Agents

49
Q

Viruses that causes yellow fever, dengue, influenza and SARS

A

Category C Agents

50
Q
  1. Hand Hygiene
  2. Gown
  3. Respiratory Protection
  4. Eye Protection
  5. Gloves
A

Donning Your PPE

51
Q
  1. Gloves
  2. Eye Protection
  3. Gown
  4. Respiratory
    5.Hand Hygiene.
A

Doffing your PPE

52
Q

Substances which singly or in combination have a significant threat or hazard to human health or to the environment and require special handling.

A

Hazardous Wastes

53
Q

Should be calibrated against a reference thermometer from the National Institute of Standards and Technology (NIST).

A

Thermometer Calibration

54
Q

Thermometer that differ by ___ from the reference thermometer should be disposed.

A

> 1degree C

55
Q

Thermometer should be checked ___ for the presence of gas bubbles.

56
Q

Immersed in glycerol. (Prevents fluctuations when door is opened)

A

Thermometer Calibration

57
Q

The temperature used for calibration are:

A

-20 °C, 2°C to 8°C, 37 °C, and 56°C

58
Q

The percentage of carbon dioxide must be checked ____.

59
Q

The speed or revolution per minute (rpm) must be checked _____ using a tachometer.

A

Checked twice a year

60
Q

culture media can be checked based on their performance and sterility., and

A

records should be kept for at least two years.

61
Q

Media that are not quality-controlled by the laboratory must still
undergo observation for

A

moisture, sterility, breakage and
appearance.

62
Q

reagent should be tested daily with both positive and negative controls.

63
Q

Antimicrobial Susceptibility uses specific strains of microorganisms from ____.

A

American Type Culture Collection (ATCC)

64
Q

Antimicrobial solutions must ______ after thawing.

A

not be frozen

65
Q

A stock culture should be grown in large volume of broth and then divided into _____ to last for a year.

66
Q

To maintain viability: it should be ____ after thawing.

A

Sub- Cultured twice

67
Q
  1. Stock Cultured Organisms stored in freezer should be kept at ____
A

-70 degrees C

68
Q

All tests performed on patients must be subjected to proficiency testing

A

Twice a year

BACTERIOLOGY P1 (3 decks)

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